Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human placenta tissue, human kidney tissue, human liver tissue, human spleen tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17082-1-AP targets RPL24 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7085 Product name: Recombinant human RPL24 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-157 aa of BC000690 Sequence: MKVELCSFSGYKIYPGHGRRYARTDGKVFQFLNAKCESAFLSKRNPRQINWTVLYRRKHKKGQSEEIQKKRTRRAVKFQRAITGASLADIMAKRNQKPEVRKAQREQAIRAAKEAKKAKQASKKTAMAAAKAPTKAAPKQKIVKPVKVSAPRVGGKR Predict reactive species |
| Full Name | ribosomal protein L24 |
| Calculated Molecular Weight | 18 kDa |
| Observed Molecular Weight | 21-23 kDa |
| GenBank Accession Number | BC000690 |
| Gene Symbol | RPL24 |
| Gene ID (NCBI) | 6152 |
| RRID | AB_2181728 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P83731 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL24 is one component of the large (60S) subunits that promote the translation of uORF-containing mRNAsgene The mutation in Rpl24 result in impairment of mRNA splicing and L24 production, which in turn affects ribosome biogenesis, protein synthesis and the cell cycle. PMID:20799971]. Also RPL24 (ribosomal protein L24) is a key factor for translation reinitiation of downstream ORFs on the polycistronic cauliflower mosaic virus 35S RNA transcription unit, and may have a role in gynoecuim development.[PMID:15270688]
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPL24 antibody 17082-1-AP | Download protocol |
| IHC protocol for RPL24 antibody 17082-1-AP | Download protocol |
| IP protocol for RPL24 antibody 17082-1-AP | Download protocol |
| WB protocol for RPL24 antibody 17082-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPL24 Polyclonal antibody (Cat# 17082-1-AP) has 28 citations published in journals including Cell, Nature Metabolism, Nature Neuroscience, Nature Communications, Neuron, PNAS, Cell Stem Cell, Cell Reports, Nucleic Acids Research, Science Advances, and others. Research fields span ribosome biology, mRNA translation and transport, neurodegeneration (Parkinson's disease, ALS/FTD), cancer (breast, cervical, laryngeal), stress granules, exosome proteomics, immunology, and reproductive biology.
| Species | Application | Title |
|---|---|---|
Cell Ribosome ADP-ribosylation inhibits translation and maintains proteostasis in cancers.
| ||
Nat Metab N6-methyladenosine (m6A) in 18S rRNA promotes fatty acid metabolism and oncogenic transformation | ||
Nat Aging Pre-symptomatic Parkinson's disease blood test quantifying repetitive sequence motifs in transfer RNA fragments | ||
Cell Stem Cell HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis. | ||
Nat Neurosci Messenger RNA transport on lysosomal vesicles maintains axonal mitochondrial homeostasis and prevents axonal degeneration |
Reviews
What customers say
One reviewer (rated 4/5) reported excellent performance for Western Blot in both human HEK and mouse MEF cells using a 1:2000 dilution in 2% BSA, noting consistent and reliable results. However, the antibody performed less well for immunoprecipitation. Overall, the review highlights strong WB performance with a minor limitation in IP applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Erica (Verified Customer) (05-15-2019) | The antibody works very well for WB in both Human HEK and MEF cells. I used 1:2000 dilution in 2%BSA, and it always work well. It doesn't work very well for IP though..
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