RPL35 Polyclonal antibody

RPL35 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14826-1-AP

Synonyms

ribosomal protein L35, Large ribosomal subunit protein uL29, 60S ribosomal protein L35

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Validation Data Gallery

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  WB analysis of mouse heart using 14826-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of mouse heart using 14826-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse skeletal muscle using 14826-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse skeletal muscle using 14826-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 14826-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 14826-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IHC staining of human liver cancer using 14826-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14826-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14826-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 14826-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 14826-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 14826-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 14826-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 14826-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14826-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14826-1-AP (RPL35 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, HeLa cells, mouse skeletal muscle tissue
• Positive IHC detected in: rat pancreas tissue, human liver cancer tissue, human pancreas cancer tissue, human stomach cancer tissue, human thyroid cancer tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14826-1-AP targets RPL35 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL35 fusion protein Ag6615
• Full Name: ribosomal protein L35
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC000348
• Gene Symbol: RPL35
• Gene ID (NCBI): 11224
• RRID: AB_2878083
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Cell Sci; Drp1 regulates transcription of ribosomal protein genes in embryonic hearts.; Authors - Qiancong Zhao