mouse heart tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of mouse heart using 14826-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of mouse skeletal muscle using 14826-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of mouse skeletal muscle using 14826-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of HeLa using 14826-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
HeLa cells were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of HeLa using 14826-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
HeLa cells were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
IHC staining of human liver cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat pancreas using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14826-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14826-1-AP (RPL35 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14826-1-AP (RPL35 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
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mouse heart tissue, HeLa cells, mouse skeletal muscle tissue
Positive IHC detected in
rat pancreas tissue, human liver cancer tissue, human pancreas cancer tissue, human stomach cancer tissue, human thyroid cancer tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:200-1:1000
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of mouse heart using 14826-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of mouse skeletal muscle using 14826-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of mouse skeletal muscle using 14826-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of HeLa using 14826-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
WB analysis of HeLa using 14826-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14826-1-AP (RPL35 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
IHC Figures
IHC staining of human liver cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thyroid cancer using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat pancreas using 14826-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 14826-1-AP (RPL35 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HeLa using 14826-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14826-1-AP (RPL35 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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