RUNX1/AML1 Recombinant monoclonal antibody

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  WB analysis of THP-1 using 85325-2-RR

  Non-treated THP-1 cells and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 85325-2-RR (RUNX1/AML1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

RUNX1/AML1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85325-2-RR

Clone No.251044E2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

RUNX1, AML1, AML1 EVI 1, AMLCR1, CBF alpha 2

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Tested Applications

• Positive WB detected in: PMA treated THP-1 cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85325-2-RR targets RUNX1/AML1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: runt-related transcription factor 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: NM_001754
• Gene Symbol: RUNX1
• Gene ID (NCBI): 861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q01196
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Runt-related transcription factor 1 (RUNX1), also named AML1 or CBF alpha 2, is a 453 amino acid protein which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except the brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in the diversification of sensory neurons. RUNX1 exists in some isoforms with a range of MW 20-52 kDa. The calculated molecular weight of isoform 1 is 49 kDa; however, the modified protein ranges from approximately 49 to 55 kDa.