RUNX1/AML1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of THP-1 using 85325-2-RR (same clone as 85325-2-PBS)

  Non-treated THP-1 cells and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 85325-2-RR (RUNX1/AML1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85325-2-PBS in a different storage buffer formulation.

RUNX1/AML1 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 85325-2-PBS

Clone No.251044E2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

RUNX1, AML1, AML1 EVI 1, AMLCR1, CBF alpha 2

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Product Information

85325-2-PBS targets RUNX1/AML1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: runt-related transcription factor 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: NM_001754
• Gene Symbol: RUNX1
• Gene ID (NCBI): 861
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q01196
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Runt-related transcription factor 1 (RUNX1), also named AML1 or CBF alpha 2, is a 453 amino acid protein which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except the brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in the diversification of sensory neurons. RUNX1 exists in some isoforms with a range of MW 20-52 kDa. The calculated molecular weight of isoform 1 is 49 kDa; however, the modified protein ranges from approximately 49 to 55 kDa.