Validation Data Gallery
|Positive WB detected in||Jurkat cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human colon cancer tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 25315-1-AP is RUNX1 (middle) Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RUNX1 (middle) fusion protein Ag17838|
|Full Name||runt-related transcription factor 1|
|Calculated molecular weight||480 aa, 52 kDa|
|Observed molecular weight||48-55 kDa|
|GenBank accession number||BC136381|
|Gene ID (NCBI)||861|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Runt-related transcription factor 1 (RUNX1), also named as AML1 or CBF alpha 2, is 453 amino acid protein, which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in sensory neuron diversification. RUNX1 exists some isoforms with range of MV 20-52 kDa. The calculated molecular weight of isoforms 1 is 49 kDa, but the modified protein is about 49-55 kDa.
|Product Specific Protocols|
|WB protocol for RUNX1 (middle) antibody 25315-1-AP||Download protocol|
|IHC protocol for RUNX1 (middle) antibody 25315-1-AP||Download protocol|
|IF protocol for RUNX1 (middle) antibody 25315-1-AP||Download protocol|
|IP protocol for RUNX1 (middle) antibody 25315-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Pharmacologic targeting of the P-TEFb complex as a therapeutic strategy for chronic myeloid leukemia.
Angiotensin II-Induced Long Non-Coding RNA Alivec Regulates Chondrogenesis in Vascular Smooth Muscle Cells.
J Cell Physiol
Inhibition of RUNX1 blocks the differentiation of lung fibroblasts to myofibroblasts.
J Hematol Oncol
RUNX1 mutations promote leukemogenesis of myeloid malignancies in ASXL1-mutated leukemia.
ASCL1 represses a SOX9+ neural crest stem-like state in small cell lung cancer.
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