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  • KD/KO Validated

RUNX1 (middle) Polyclonal antibody

RUNX1 (middle) Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 25315-1-AP


AML1, AML1 EVI 1, AMLCR1, CBF alpha 2, CBFA2, EVI 1, Oncogene AML 1, PEA2 alpha B, PEBP2 alpha B, PEBP2aB, RUNX1, RUNX1 (middle)

Tested Applications

Positive WB detected inJurkat cells, mouse thymus tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inJurkat cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25315-1-AP targets RUNX1 (middle) in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RUNX1 (middle) fusion protein Ag17838
Full Name runt-related transcription factor 1
Calculated Molecular Weight 480 aa, 52 kDa
Observed Molecular Weight 48-55 kDa
GenBank Accession NumberBC136381
Gene Symbol RUNX1
Gene ID (NCBI) 861
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Runt-related transcription factor 1 (RUNX1), also named AML1 or CBF alpha 2, is a 453 amino acid protein, which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except the brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in sensory neuron diversification. RUNX1 exists in some isoforms with a range of MV 20-52 kDa. The calculated molecular weight of isoform 1 is 49 kDa, but the modified protein is about 49-55 kDa.


Product Specific Protocols
WB protocol for RUNX1 (middle) antibody 25315-1-APDownload protocol
IHC protocol for RUNX1 (middle) antibody 25315-1-APDownload protocol
IF protocol for RUNX1 (middle) antibody 25315-1-APDownload protocol
IP protocol for RUNX1 (middle) antibody 25315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation.

Authors - Dapeng Wang
mouseIHC, WB

Genes Dev

ASCL1 represses a SOX9+ neural crest stem-like state in small cell lung cancer.

Authors - Rachelle R Olsen

J Hematol Oncol

RUNX1 mutations promote leukemogenesis of myeloid malignancies in ASXL1-mutated leukemia.

Authors - Rabindranath Bera

Int Immunopharmacol

miR-9 targeting RUNX1 improves LPS-induced alveolar hypercoagulation and fibrinolysis inhibition through NF-κB inactivation in ARDS

Authors - Chuan Xiao

J Cell Physiol

Inhibition of RUNX1 blocks the differentiation of lung fibroblasts to myofibroblasts.

Authors - Shubham Dubey
  • KD Validated

Eur J Pharmacol

Vitamin D3 analogue calcipotriol inhibits the profibrotic effects of transforming growth factor- β1 on pancreatic stellate cells

Authors - Meifang Zheng


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


SD (Verified Customer) (07-08-2022)

Excellent Antibody!

  • Applications: Western Blot
  • Cell Tissue Type: H9C2
RUNX1 (middle) Antibody Western Blot validation ( dilution) in H9C2 (Cat no:25315-1-AP)

P (Verified Customer) (12-01-2021)


  • Applications: Western Blot
  • Cell Tissue Type: fibroblast
RUNX1 (middle) Antibody Western Blot validation ( dilution) in fibroblast (Cat no:25315-1-AP)