RUNX1 (middle) Polyclonal antibody

• Featured Product
• KD/KO Validated

RUNX1 (middle) Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

15

Reviews (2)

Cat no : 25315-1-AP

Synonyms

AML1, AML1 EVI 1, AMLCR1, CBF alpha 2, CBFA2, EVI 1, Oncogene AML 1, PEA2 alpha B, PEBP2 alpha B, PEBP2aB, RUNX1, RUNX1 (middle)

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Validation Data Gallery

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  WB analysis using 25315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25315-1-AP (RUNX1 (middle) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25315-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25315-1-AP (RUNX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 25315-1-AP

  IP result of anti-RUNX1 (middle) (IP:25315-1-AP, 4ug; Detection:25315-1-AP 1:300) with Jurkat cells lysate 3440ug.

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  IHC staining of human colon cancer using 25315-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25315-1-AP (RUNX1 (middle) antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 25315-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25315-1-AP (RUNX1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 25315-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25315-1-AP (RUNX1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 25315-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25315-1-AP (RUNX1 (middle) antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 25315-1-AP

  Immunofluorescent analysis of HepG2 cells using 25315-1-AP (RUNX1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Jurkat using 25315-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human RUNX1 (middle) (25315-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse thymus tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human colon cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

25315-1-AP targets RUNX1 (middle) in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RUNX1 (middle) fusion protein Ag17838
• Full Name: runt-related transcription factor 1
• Calculated Molecular Weight: 480 aa, 52 kDa
• Observed Molecular Weight: 48-55 kDa
• GenBank Accession Number: BC136381
• Gene Symbol: RUNX1
• Gene ID (NCBI): 861
• RRID: AB_2880026
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Runt-related transcription factor 1 (RUNX1), also named AML1 or CBF alpha 2, is a 453 amino acid protein, which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except the brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in sensory neuron diversification. RUNX1 exists in some isoforms with a range of MV 20-52 kDa. The calculated molecular weight of isoform 1 is 49 kDa, but the modified protein is about 49-55 kDa.

Publications

Species	Application	Title
mouse	WB	Immunity; The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation.; Authors - Dapeng Wang
mouse	IHC, WB	Genes Dev; ASCL1 represses a SOX9+ neural crest stem-like state in small cell lung cancer.; Authors - Rachelle R Olsen
human	WB	J Hematol Oncol; RUNX1 mutations promote leukemogenesis of myeloid malignancies in ASXL1-mutated leukemia.; Authors - Rabindranath Bera
rat	WB	Int Immunopharmacol; miR-9 targeting RUNX1 improves LPS-induced alveolar hypercoagulation and fibrinolysis inhibition through NF-κB inactivation in ARDS; Authors - Chuan Xiao
human	WB	J Cell Physiol; Inhibition of RUNX1 blocks the differentiation of lung fibroblasts to myofibroblasts.; Authors - Shubham Dubey ; KD Validated
human	WB	Eur J Pharmacol; Vitamin D3 analogue calcipotriol inhibits the profibrotic effects of transforming growth factor- β1 on pancreatic stellate cells; Authors - Meifang Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SD (Verified Customer) (07-08-2022)

Excellent Antibody!

• Applications: Western Blot
• Cell Tissue Type: H9C2

RUNX1 (middle) Antibody Western Blot validation ( dilution) in H9C2 (Cat no:25315-1-AP)

FH

P (Verified Customer) (12-01-2021)

Excellent

• Applications: Western Blot
• Cell Tissue Type: fibroblast

RUNX1 (middle) Antibody Western Blot validation ( dilution) in fibroblast (Cat no:25315-1-AP)