RUNX1 (middle) Polyclonal antibody

RUNX1 (middle) Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25315-1-AP

Synonyms

AML1, AML1 EVI 1, AMLCR1, CBF alpha 2, CBFA2, EVI 1, Oncogene AML 1, PEA2 alpha B, PEBP2 alpha B, PEBP2aB, RUNX1



Tested Applications

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25315-1-AP is RUNX1 (middle) Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RUNX1 (middle) fusion protein Ag17838
Full Name runt-related transcription factor 1
Calculated molecular weight 480 aa, 52 kDa
Observed molecular weight 48-55 kDa
GenBank accession numberBC136381
Gene symbol RUNX1
Gene ID (NCBI) 861
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Runt-related transcription factor 1 (RUNX1), also named as AML1 or CBF alpha 2, is 453 amino acid protein, which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in sensory neuron diversification. RUNX1 exists some isoforms with range of MV 20-52 kDa. The calculated molecular weight of isoforms 1 is 49 kDa, but the modified protein is about 49-55 kDa.

Protocols

Product Specific Protocols
WB protocol for RUNX1 (middle) antibody 25315-1-APDownload protocol
IHC protocol for RUNX1 (middle) antibody 25315-1-APDownload protocol
IF protocol for RUNX1 (middle) antibody 25315-1-APDownload protocol
IP protocol for RUNX1 (middle) antibody 25315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Cancer Cell Int

Identification of lysine acetylome in cervical cancer by label-free quantitative proteomics.

Authors - Lu Zhang
humanWB

Cell Commun Signal

Pharmacologic targeting of the P-TEFb complex as a therapeutic strategy for chronic myeloid leukemia.

Authors - Yingjie Qing
humanWB

J Hematol Oncol

RUNX1 mutations promote leukemogenesis of myeloid malignancies in ASXL1-mutated leukemia.

Authors - Rabindranath Bera
mouseIHC, WB

Genes Dev

ASCL1 represses a SOX9+ neural crest stem-like state in small cell lung cancer.

Authors - Rachelle R Olsen
mouseWB

Immunity

The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation.

Authors - Dapeng Wang