RUVBL1 Polyclonal antibody, PBS Only

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  WB analysis using 10210-2-AP (same clone as 10210-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10210-2-AP (RUVBL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 10210-2-AP (same clone as 10210-2-PBS)

  IP result of anti-RUVBL1 (IP:10210-2-AP, 3ug; Detection:10210-2-AP 1:1000) with HeLa cells lysate 800ug. This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 10210-2-AP (same clone as 10210-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10210-2-AP (RUVBL1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 10210-2-AP (same clone as 10210-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10210-2-AP (RUVBL1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 10210-2-AP (same clone as 10210-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10210-2-AP (RUVBL1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 10210-2-AP (same clone as 10210-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10210-2-AP (RUVBL1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 10210-2-AP (same clone as 10210-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RUVBL1 antibody (10210-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10210-2-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RUVBL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10210-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

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Product Information

10210-2-PBS targets RUVBL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0281

  Product name: Recombinant human RUVBL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-214 aa of BC002993

  Sequence: MKIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAGPPGTGKTALALAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEVYEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESLQKERVEAGDVIYIEANSGAVKRQGRCDTYATEFD

• Full Name: RuvB-like 1 (E. coli)
• Calculated Molecular Weight: 456 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC002993
• Gene Symbol: RUVBL1
• Gene ID (NCBI): 8607
• RRID: AB_2184405
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y265
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198]. This antibody is a rabbit polyclonal antibody to human RUVBL1.