Rat IL-1 beta ELISA Kit
Cat no : KE20021
Synonyms
Il1 beta, Il1b, IL 1 beta, IL-1 beta,IL 1 beta,IL1beta, IL-1 β
Validation Data Gallery
Product Information
KE20021 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat IL-1 beta ELISA kit is to be used to detect and quantify protein levels of endogenous rat IL-1 beta. The assay recognizes rat IL-1 beta. An antibody specific for rat IL-1 beta has been pre-coated onto the microwells. The rat IL-1 beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for rat IL-1 beta is added to detect the captured rat IL-1 beta protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
| Product name | Rat IL-1 beta ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.9 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 24494 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat Serum | 114% | 102%-127% |
| Cell culture supernatant | 97% | 70%-128% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 470.7 | 20.4 | 4.3 |
| 2 | 20 | 112.8 | 4.2 | 3.7 |
| 3 | 20 | 28.8 | 1.2 | 4.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 493.8 | 38.3 | 7.8 |
| 2 | 24 | 110.0 | 5.4 | 4.9 |
| 3 | 24 | 27.1 | 1.5 | 5.6 |
Background Information
Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il1 beta, Il1b, IL 1 beta, IL-1 beta,IL 1 beta,IL1beta, IL-1 β |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Cell Commun Signal Spinster homolog 2/S1P signaling ameliorates macrophage inflammatory response to bacterial infections by balancing PGE2 production | ||
Int Immunopharmacol Bardoxolone methyl breaks the vicious cycle between M1 macrophages and senescent nucleus pulposus cells through the Nrf2/STING/NF-κB pathway | ||
J Ginseng Res Panax ginseng alleviates diabetic peripheral neuropathy by suppressing mitochondrial dysfunction and oxidative stress via modulation of the RAGE, NF-κB, and Nrf2 pathways. | ||
Ann Med FOXG1 interaction with SATB2 promotes autophagy to alleviate neuroinflammation and mechanical abnormal pain in rats with lumbar disc herniation | ||
BMC Complement Med Ther Exploring the protective role of green tea extract against cardiovascular alterations induced by chronic REM sleep deprivation via modulation of inflammation and oxidative stress | ||
FASEB J ARIH1 Promotes Preeclampsia by Inducing MFN2-Dependent Hypoxia-Triggered Mitophagy and Endoplasmic Reticulum Stress in Trophoblasts. |