Rat Lipocalin-2/NGAL ELISA Kit
Cat no : KE20034
Synonyms
Lcn2,Alpha-2-microglobulin-related protein,Alpha-2
Validation Data Gallery
Product Information
KE20034 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The rat Lipocalin-2/NGAL ELISA kit is to be used to detect and quantify protein levels of endogenous rat Lipocalin-2/NGAL. The assay recognizes rat Lipocalin-2/NGAL. An antibody specific for rat Lipocalin-2/NGAL has been pre-coated onto the microwells. The rat Lipocalin-2/NGAL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for rat Lipocalin-2/NGAL is added to detect the captured rat Lipocalin-2/NGAL protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Rat Lipocalin-2/NGAL ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 1.8 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 170496 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 103% | 98%-109% |
| Tissue lysate | 105% | 99%-111% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 136.1 | 2.3 | 1.7 |
| 2 | 20 | 48.7 | 0.7 | 1.5 |
| 3 | 20 | 22.0 | 0.6 | 2.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 136.3 | 3.4 | 2.5 |
| 2 | 24 | 49.0 | 0.7 | 1.5 |
| 3 | 24 | 21.7 | 0.6 | 2.7 |
Background Information
Neutrophil gelatinase-associated lipocalin (NGAL) also known as Lipocalin-2 (LCN2), is a secreted glycoprotein that belongs to the lipocalin protein family. Several roles of NGAL were identified, including iron trafficking, chemotactic, and promoting cell differentiation and proliferation. NGAL was originally found in neutrophils and expressed in tissues containing renal, uterus, liver, spleen, cardiomyocytes, neurons, and immune cells. NGAL is a significant biomarker of cardiovascular diseases, ischemic stroke, acute renal injury, inflammation, and metabolic diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Lcn2,Alpha-2-microglobulin-related protein,Alpha-2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.