S1PR1/EDG1 Polyclonal antibody

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  WB analysis using 55133-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55133-1-AP (S1PR1/EDG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55133-1-AP

  IP result of anti-S1PR1/EDG1 (IP:55133-1-AP, 4ug; Detection:55133-1-AP 1:300) with mouse brain tissue lysate 2640ug.

• Featured Product
• KD/KO Validated

S1PR1/EDG1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 55133-1-AP

Publications(49)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

S1PR1, EDG1, EDG 1, ECGF1, D1S3362

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 44 publications below
• IHC: See 10 publications below
• IF: See 12 publications below
• IP: See 4 publications below

Product Information

55133-1-AP targets S1PR1/EDG1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: sphingosine-1-phosphate receptor 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: NM_001400
• Gene Symbol: S1PR1
• Gene ID (NCBI): 1901
• RRID: AB_10793721
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P21453
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S1PR1 (sphingosine-1-phosphate receptor 1), also known as S1P1 or EDG1, is one of five G-protein-coupled receptors for sphingosine-1-phosphate (S1P) (PMID: 18787560). S1P1 and its receptors have important regulatory functions in normal physiology and disease processes, particularly involving the immune, central nervous, and cardiovascular systems (PMID: 21339489). S1PR1 is widely expressed in various cell types, including endothelial cells and lymphocytes (PMID: 14737169). S1P-S1PR1 plays a major role in lymphocyte egress and chemotaxis, cell proliferation and survival, and tumor angiogenesis and metastasis (PMID: 21102457). The antibody is specific to S1PR1.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	BMC Med; Involvement of sphingosine-1-phosphate receptor 1 in pain insensitivity in a BTBR mouse model of autism spectrum disorder; Authors - Lili Fan
rat	IF	Neural Regen Res; Fingolimod protects against neurovascular unit injury in a rat model of focal cerebral ischemia/reperfusion injury; Authors - Xiao-Yu Zhu
mouse	WB	iScience; Global sphingosine-1-phosphate receptor 2 deficiency attenuates neuroinflammation and ischemic-reperfusion injury after neonatal stroke; Authors - Yumi Fukuzaki
rat	WB	Int Immunopharmacol; Dexmedetomidine alleviates oxidative stress and mitochondrial dysfunction in diabetic peripheral neuropathy via the microRNA-34a/SIRT2/S1PR1 axis; Authors - Ying Lin
human	IHC, WB, IP	Front Cell Dev Biol; The CAGE-MiR-181b-5p-S1PR1 Axis Regulates Anticancer Drug Resistance and Autophagy in Gastric Cancer Cells.; Authors - Minjeong Yeon
rat	WB	Front Pharmacol; Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.; Authors - Han-Qing Pang