SAMHD1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86798-1-RR (same clone as 86798-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86798-1-RR (SAMHD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86798-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 86798-1-RR (same clone as 86798-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SAMHD1 antibody (86798-1-RR, Clone: 251815H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86798-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86798-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86798-1-RR against Human SAMHD1 were performed. The affinity constant is 0.851 nM.

SAMHD1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86798-1-PBS

Clone No.251815H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DCIP, Dendritic cell-derived IFNG-induced protein, Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, dNTPase, EC:3.1.5.-

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Product Information

86798-1-PBS targets SAMHD1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3287

  Product name: Recombinant human SAMHD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-315 aa of BC036450

  Sequence: MQRADSEQPSKRPRCDDSPRTPSNTPSAEADWSPGLELHPDYKTWGPEQVCSFLRRGGFEEPVLLKNIRENEITGALLPCLDESRFENLGVSSLGERKKLLSYIQRLVQIHVDTMKVINDPIHGHIELHPLLVRIIDTPQFQRLRYIKQLGGGYYVFPGASHNRFEHSLGVGYLAGCLVHALGEKQPELQISERDVLCVQIAGLCHDLGHGPFSHMFDGRFIPLARPEVKWTHEQGSVMMFEHLINSNGIKPVMEQYGLIPEEDICFIKEQIVGPLESPVEDSLWPYKGRPENKSFLYEIVSNKRNGIDVDKWDY

• Full Name: SAM domain and HD domain 1
• Calculated Molecular Weight: 626 aa, 72 kDa
• Observed Molecular Weight: 64-72 kDa
• GenBank Accession Number: BC036450
• Gene Symbol: SAMHD1
• Gene ID (NCBI): 25939
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y3Z3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with presumed immune pathogenesis. Three alternatively spliced transcripts encoding different isoforms have been described.