Tested Applications
| Positive WB detected in | rat liver tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16246-1-AP targets SAT2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9179 Product name: Recombinant human SAT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-170 aa of BC011751 Sequence: MASVRIREAKEGDCGDILRLIRELAEFEKLSDQVKISEEALRADGFGDNPFYHCLVAEILPAPGKLLGPCVVGYGIYYFIYSTWKGRTIYLEDIYVMPEYRGQGIGSKIIKKVAEVALDKGCSQFRLAVLDWNQRAMDLYKALGAQDLTEAEGWHFFCFQGEATRKLAGK Predict reactive species |
| Full Name | spermidine/spermine N1-acetyltransferase family member 2 |
| Calculated Molecular Weight | 170 aa, 19 kDa |
| Observed Molecular Weight | 19 kDa |
| GenBank Accession Number | BC011751 |
| Gene Symbol | SAT2 |
| Gene ID (NCBI) | 112483 |
| RRID | AB_2254874 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96F10 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Spermidine/spermine N1-acetyltransferase 2 (SAT2) is a short-lived polyamine catabolic enzyme inducible by polyamines and polyamine analogues. Human SAT2 contains the highly conserved acetyl-CoA-binding domain and displays coactivator function to enhance NF-κB-dependent transcription and enhances TNFα-induced NF-κB activity (PMID: 17875644). Human SAT1 and SAT2 proteins, which share 46% amino acid sequence identity and are clearly homologous, both interact with HIF-1α and both promote the ubiquitination and degradation of HIF-1α (PMID: 17011643).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SAT2 antibody 16246-1-AP | Download protocol |
| WB protocol for SAT2 antibody 16246-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SAT2 antibody (Cat# 16246-1-AP) has 5 citations across Free Radical Biology and Medicine, Life Sciences, Neuropharmacology, Current Issues in Molecular Biology, and Toxicology in Vitro. Research fields include ferroptosis in pulmonary arterial hypertension, diabetic nephropathy, ketamine's antidepressant mechanisms, pancreatic cancer tumor suppression via PI3K/Akt-MAPK pathways, and busulfan-induced testicular Sertoli cell injury.
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med Therapeutic potential of magnesium diboride nanosheets in PAH-associated right heart failure: Integrated multi-omics analysis reveals ferroptosis suppression via LC3/ATG5/NCOA4/FTH1 pathway. | ||
Life Sci KAT2A-mediated H3K79 succinylation promotes ferroptosis in diabetic nephropathy by regulating SAT2 | ||
Neuropharmacology A role of GABAA receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine | ||
Curr Issues Mol Biol The Tumor-Suppressive Role of SAT2 in Pancreatic Cancer: Involvement in PI3K/Akt-MAPK Pathways and Immune Modulation. | ||
Toxicol In Vitro Increased Sat2 expression is associated with busulfan-induced testicular Sertoli cell injury. |
