Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | mouse bladder tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15288-1-AP targets SC65 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7480 Product name: Recombinant human SC65 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 92-437 aa of BC001047 Sequence: DGGRADEWACELRLFGRVLERAACLRRCKRTLPAFQVPYPPRQLLRDFQSRLPYQYLHYALFKANRLEKAVAAAYTFLQRNPKHELTAKYLNYYQGMLDVADESLTDLEAQPYEAVFLRAVKLYNSGDFRSSTEDMERALSEYLAVFARCLAGCEGAHEQVDFKDFYPAIADLFAESLQCKVDCEANLTPNVGGYFVDKFVATMYHYLQFAYYKLNDVRQAARSAASYMLFDPKDSVMQQNLVYYRFHRARWGLEEEDFQPREEAMLYHNQTAELRELLEFTHMYLQSDDEMELEETEPPLEPEDALSDAEFEGEGDYEEGMYADWWQEPDAKGDEAEAEPEPELA Predict reactive species |
| Full Name | synaptonemal complex protein SC65 |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC001047 |
| Gene Symbol | SC65 |
| Gene ID (NCBI) | 10609 |
| RRID | AB_2184611 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92791 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SC65, also named as Leprecan-like protein 4 or Nucleolar autoantigen No55, is a 437 amino acid protein, which belongs to the leprecan family. SC65 ia a protein of the interphase nucleolus and tumour-associated autoantigen in patients with prostate cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for SC65 antibody 15288-1-AP | Download protocol |
| WB protocol for SC65 antibody 15288-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody is anti-P3H4 (Sc65), catalog number 15288-1-AP, with a total of 7 citations. These appear in journals including Journal of Bone and Mineral Research, Aging, Scientific Reports, Cancers, PLoS Genetics, and AIMS Genetics. The associated research fields span bone mass homeostasis, bladder and lung cancer progression, congestive heart failure, collagen lysyl hydroxylation, and post-translational modifications of collagen. Applications cited include Western blot, immunofluorescence, and IHC.
| Species | Application | Title |
|---|---|---|
J Bone Miner Res Sc65 is a novel endoplasmic reticulum protein that regulates bone mass homeostasis.
| ||
Aging (Albany NY) Knockdown of P3H4 inhibits proliferation and invasion of bladder cancer.
| ||
Sci Rep Transcriptome analysis of a dog model of congestive heart failure shows that collagen-related 2-oxoglutarate-dependent dioxygenases contribute to heart failure | ||
Cancers (Basel) P3H4 Promotes Malignant Progression of Lung Adenocarcinoma via Interaction with EGFR.
| ||
PLoS Genet Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.
| ||
PLoS Genet Cyclophilin B control of lysine post-translational modifications of skin type I collagen. |
