SCAPER Polyclonal antibody

SCAPER Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16994-1-AP

Synonyms

KIAA1454, MSTP063, SCAPER, Zfp291, Zinc finger protein 291, ZNF291

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Validation Data Gallery

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  IHC staining of mouse testis using 16994-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16994-1-AP (SCAPER antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 16994-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16994-1-AP (SCAPER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 16994-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16994-1-AP (SCAPER Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16994-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16994-1-AP (SCAPER Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: mouse testis tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 16994-1-AP is SCAPER Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCAPER fusion protein Ag10679
• Full Name: S-phase cyclin A-associated protein in the ER
• Calculated Molecular Weight: 292 aa, 32 kDa
• GenBank Accession Number: BC015212
• Gene Symbol: SCAPER
• Gene ID (NCBI): 49855
• RRID: AB_2878339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCAPER, also named as KIAA1454 and ZNF291, is a CCNA2/CDK2 regulatory protein that transiently maintains CCNA2 in the cytoplasm.

Publications

Species	Application	Title
mouse	IHC	J Med Genet; Mutations in SCAPER cause autosomal recessive retinitis pigmentosa with intellectual disability.; Authors - Yasmin Tatour
mouse	IF	Hum Mol Genet; Male Sterility and Reduced Female Fertility in SCAPER-Deficient Mice.; Authors - Yasmin Tatour