SCLY Polyclonal antibody

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  WB analysis using 10667-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10667-1-AP (SCLY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SCLY Polyclonal Antibody for WB, ELISA

Cat No. 10667-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

hSCL, SCL, SCLY, selenocysteine lyase

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10667-1-AP targets SCLY in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCLY fusion protein Ag0972
• Full Name: selenocysteine lyase
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC007891
• Gene Symbol: SCLY
• Gene ID (NCBI): 51540
• RRID: AB_2183693
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96I15
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Selenocysteine lyase(SCLY or SCL) catalyzes the decomposition of L-selenocysteine to L-alanine and elemental selenium. This protein expresses in all mouse tissues examined, with the highest expression in liver, kidney, and testis. Mouse Scly localized to the cytosolic fraction in mouse liver and formed homodimers(PMID:10692412).This antibody detects the N-terminal of the protein.