SCN9A/Nav1.7-Specific Polyclonal antibody

SCN9A/Nav1.7-Specific Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 20257-1-AP

Synonyms

Nav1.7, SCN9A, NENA, NE NA, hNE-Na

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Validation Data Gallery

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  IHC staining of human brain using 20257-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20257-1-AP (SCN9A/Nav1.7-Specific antibody at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 5 publications below

Product Information

20257-1-AP targets SCN9A/Nav1.7-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: sodium channel, voltage-gated, type IX, alpha subunit
• Calculated Molecular Weight: 226 kDa
• GenBank Accession Number: NM_002977
• Gene Symbol: SCN9A
• Gene ID (NCBI): 6335
• RRID: AB_11182704
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCN9A, also named as NENA, PN1, ETHA, NE-NA, Nav1.7 and hNE-Na, belongs to the sodium channel family. SCN9A mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, SCN9A forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na+ channel isoform. SCN9a plays a role in pain mechanisms, especially in the development of inflammatory pain. Defects in SCN9A are the cause of primary erythermalgia or autosomal recessive congenital indifference to pain or paroxysmal extreme pain disorder (PEPD). The antibody is specific to SCN9A

Publications

Species	Application	Title
mouse	IF	Prog Neurobiol; MicroRNA-96 is required to prevent allodynia by repressing voltage-gated sodium channels in spinal cord.; Authors - Liting Sun
rat	WB,IF	J Neuroinflammation; Glial interleukin-1β upregulates neuronal sodium channel 1.7 in trigeminal ganglion contributing to temporomandibular joint inflammatory hypernociception in rats.; Authors - Peng Zhang
rat	WB	Inflammation; Prostaglandin E2 Upregulated Trigeminal Ganglionic Sodium Channel 1.7 Involving Temporomandibular Joint Inflammatory Pain in Rats.; Authors - Peng Zhang
rat	WB	Chin J Dent Res; Non-steroidal Anti-inflammatory Drugs Attenuate Hyperalgesia and Block Upregulation of Trigeminal Ganglionic Sodium Channel 1.7 after Induction of Temporomandibular Joint Inflammation in Rats.; Authors - Rui Yun Bi
rat	WB,IF	Neurosci Lett; Connexin 43 contributes to temporomandibular joint inflammation induced-hypernociception via sodium channel 1.7 in trigeminal ganglion.; Authors - Yi-Zhou Jin
mouse	IF	Front Mol Neurosci; Ectopic expression of Nav1.7 in spinal dorsal horn neurons induced by NGF contributes to neuropathic pain in a mouse spinal cord injury model; Authors - Yan Fu