SCN9A/Nav1.7-Specific Polyclonal antibody

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• SCN9A/Nav1.7-Specific Polyclonal antibody (20257-1-AP)

SCN9A/Nav1.7-Specific Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20257-1-AP Copied!

Publications (11)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Nav1.7, SCN9A, ETHA, hNE Na, hNE-Na

• 

  WB analysis using 20257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20257-1-AP (SCN9A/Nav1.7-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 20257-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20257-1-AP (SCN9A/Nav1.7-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 20257-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20257-1-AP (SCN9A/Nav1.7-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 20257-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20257-1-AP (SCN9A/Nav1.7-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse brain tissue, fetal human brain tissue, mouse cerebellum tissue, pig brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20257-1-AP targets SCN9A/Nav1.7-Specific in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: sodium channel, voltage-gated, type IX, alpha subunit
• Calculated Molecular Weight: 226 kDa
• Observed Molecular Weight: 226 kDa
• GenBank Accession Number: NM_002977
• Gene Symbol: SCN9A
• Gene ID (NCBI): 6335
• RRID: AB_11182704
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15858
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCN9A, also named as NENA, PN1, ETHA, NE-NA, Nav1.7 and hNE-Na, belongs to the sodium channel family. SCN9A mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, SCN9A forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na+ channel isoform. SCN9a plays a role in pain mechanisms, especially in the development of inflammatory pain. Defects in SCN9A are the cause of primary erythermalgia or autosomal recessive congenital indifference to pain or paroxysmal extreme pain disorder (PEPD). The antibody is specific to SCN9A.

Publications

What published studies show

The anti-Nav1.7 antibody (Cat# 20257-1-AP) has 11 total citations. These appear in journals including Advanced Science, Journal of Controlled Release, Journal of Neuroinflammation, EMBO Molecular Medicine, Inflammation, Neuropharmacology, Progress in Neurobiology, Frontiers in Molecular Neuroscience, Journal of Oral Biosciences, Neuroscience Letters, and Chinese Journal of Dental Research. Research fields span neuropathic pain, osteoarthritis, temporomandibular joint inflammation, and voltage-gated sodium channel biology.

Automatically generated from the 11 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IP,IF	Adv Sci (Weinh); Targeting Supramolecular Active Complexes of Na(v)1.7/Na(v)1.8 to Relieve Chronic Neuropathic Pain.; Authors - Liting Sun
rat	WB,IF	J Control Release; Ultrasound-responsive piezoelectric analgesic microspheres alleviate osteoarthritis pain.; Authors - Xinhe Li
rat	WB,IF	J Neuroinflammation; Glial interleukin-1β upregulates neuronal sodium channel 1.7 in trigeminal ganglion contributing to temporomandibular joint inflammatory hypernociception in rats.; Authors - Peng Zhang
mouse	IF	EMBO Mol Med; Cold exposure promotes the progression of osteoarthritis through downregulating APOE in cartilage; Authors - Yueqi Zhang
rat	WB	Inflammation; Prostaglandin E2 Upregulated Trigeminal Ganglionic Sodium Channel 1.7 Involving Temporomandibular Joint Inflammatory Pain in Rats.; Authors - Peng Zhang
rat	WB,IF	Neuropharmacology; Neurotransmitter Imbalance and Amygdala Synaptic Plasticity in Lumbar Disc Herniation-induced Chronic Pain and Related Emotional Disturbances:A multi-omics analysis; Authors - Zhenyu Huang