SDHB Polyclonal antibody

• Featured Product
• KD/KO Validated

SDHB Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

30

Reviews (2)

Cat no : 10620-1-AP

Synonyms

IP, PGL4, SDH, SDH1, SDHB, SDHIP

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Validation Data Gallery

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  WB analysis using 10620-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10620-1-AP (SDHB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10620-1-AP

  These five antibodies, ATP5A1 (14676-1-AP, 1:8000), UQCRC2 (14742-1-AP, 1:4000), SDHB (10620-1-AP, 1:8000), MTCO2 (55070-1-AP, 1:1000), NDUFB8 (14794-1-AP, 1:5000), can be assembled OXPHOS kit to detect the relative levels of the 5 OXPHOS complexes in mitochondrial preparations.

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  WB analysis of mouse liver using 10620-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10620-1-AP (SDHB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10620-1-AP

  IP result of anti-SDHB(IP:10620-1-AP, 4ug; Detection:10620-1-AP 1:5000) with HEK-293 cells lysate 1360 ug.

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  IHC staining of human heart using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse kidney using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse kidney using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 10620-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10620-1-AP (SDHB antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, HeLa cells, mouse liver tissue, HepG2 cells, HEK-293 cells, rat heart tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human heart tissue, human renal cell carcinoma tissue, human kidney tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 25 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

10620-1-AP targets SDHB in WB, IP, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SDHB fusion protein Ag0968
• Full Name: succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
• Calculated molecular weight: 32 kDa
• Observed molecular weight: 28-32 kDa
• GenBank accession number: BC007840
• Gene symbol: SDHB
• Gene ID (NCBI): 6390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SDHB(Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial) is also named as SDH, SDH1 and belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. The SDHD, SDHB, and SDHC genes encode subunits of mitochondrial complex II (succinate dehydrogenase). Mitochondrial complex II is a heterotetrameric complex involved in the aerobic electron transport chain and catalyses the oxidation of succinate to fumarate (Krebs cycle) with transport of electrons to the ubiquinone pool. SDHA and SDHB are the hydrophilic catalytic part of the complex and are highly conserved(J Med Genet 2004;41:e99). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS) and Cowden-like syndrome (CWDLS).

Publications

Species	Application	Title
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang ; KD Validated
mouse	WB	Diabetes; CHCHD10 Modulates Thermogenesis of Adipocytes by Regulating Lipolysis; Authors - Meng Ding
mouse	WB	Environ Pollut; Accumulation of polystyrene microplastics induces liver fibrosis by activating cGAS/STING pathway.; Authors - Rong Shen
rat	WB	Antioxid Redox Signal; Punicalagin Protects Against Diabetic Cardiomyopathy by Promoting Opa1-Mediated Mitochondrial Fusion via Regulating PTP1B-Stat3 Pathway.; Authors - Feng Fu
human	WB	Free Radic Biol Med; Mitochondrial and glucose metabolic dysfunctions in granulosa cells induce impaired oocytes of polycystic ovary syndrome through Sirtuin 3.; Authors - Qing Zhang
human	WB	J Med Genet; COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency.; Authors - Anabel Martinez Lyons

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Edward (Verified Customer) (11-15-2022)

Used with 12 ug protein lysate SDHB works quite well, both with ECL detection and fluorescent secondary antibodies it gives a strong band

• Applications: Western Blot
• Primary Antibody Dilution: 1:8000
• Cell Tissue Type: K562 and KCL22

FH

Lana (Verified Customer) (07-15-2020)

SDS-PAGE: 20 ug/ul RIPA protein lysate. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1h, room T.Primary Ab: O/N incubation at 4C, 1:5000.Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.Lines of WB image: 1 – protein ladder, 2 - whole cell lysate, 3 – mitochondrial fraction lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse embryonic stem cells

SDHB Antibody Western Blot validation (1:5000 dilution) in Mouse embryonic stem cells (Cat no:10620-1-AP)