SDHB Polyclonal antibody

• Featured Product
• KD/KO Validated

SDHB Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

15

Reviews (1)

Cat no : 10620-1-AP

Synonyms

IP, PGL4, SDH, SDH1, SDHB, SDHIP

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Validation Data Gallery

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  WB analysis using 10620-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10620-1-AP (SDHB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 10620-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10620-1-AP (SDHB Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 10620-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10620-1-AP (SDHB antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  WB analysis using 10620-1-AP

  These five antibodies, ATP5A1 (14676-1-AP, 1:8000), UQCRC2 (14742-1-AP, 1:4000), SDHB (10620-1-AP, 1:8000), MTCO2 (55070-1-AP, 1:1000), NDUFB8 (14794-1-AP, 1:5000), can be assembled OXPHOS kit to detect the relative levels of the 5 OXPHOS complexes in mitochondrial preparations.

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  IP experiment of HEK-293 using 10620-1-AP

  IP Result of anti-SDHB (IP:10620-1-AP, 4ug; Detection:10620-1-AP 1:500) with HEK-293 cells lysate 2800ug.

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Tested Applications

• Positive WB detected in: mouse heart tissue, HeLa cells, mouse liver tissue, HepG2 cells, HEK-293 cells, rat heart tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human heart tissue, human renal cell carcinoma tissue, human kidney tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

10620-1-AP targets SDHB in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SDHB fusion protein Ag0968
• Full Name: succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
• Calculated molecular weight: 32 kDa
• Observed molecular weight: 28-32 kDa
• GenBank accession number: BC007840
• Gene symbol: SDHB
• Gene ID (NCBI): 6390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SDHB(Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial) is also named as SDH, SDH1 and belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. The SDHD, SDHB, and SDHC genes encode subunits of mitochondrial complex II (succinate dehydrogenase). Mitochondrial complex II is a heterotetrameric complex involved in the aerobic electron transport chain and catalyses the oxidation of succinate to fumarate (Krebs cycle) with transport of electrons to the ubiquinone pool. SDHA and SDHB are the hydrophilic catalytic part of the complex and are highly conserved(J Med Genet 2004;41:e99). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS) and Cowden-like syndrome (CWDLS).

Publications

Species	Application	Title
mouse	WB	Autophagy; Deficiency of mitophagy receptor FUNDC1 impairs mitochondrial quality and aggravates dietary-induced obesity and metabolic syndrome.; Authors - Hao Wu
mouse	WB	Cell Death Dis; Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells.; Authors - Jun Jing
human	WB	Nat Metab; Hepcidin sequesters iron to sustain nucleotide metabolism and mitochondrial function in colorectal cancer epithelial cells.; Authors - Andrew J Schwartz
	IHC	Urology; A Renal Cell Carcinoma with Biallelic Somatic TSC2 Mutation: Clinical Study and Literature Review.; Authors - Jian Pang
human	IHC	Endocr Connect; Germline SDHB and SDHD mutations in pheochromocytoma and paraganglioma patients.; Authors - Yiqiang Huang
human	WB,IHC	Transl Oncol; SDHB deficiency promotes TGFβ-mediated invasion and metastasis of colorectal cancer through transcriptional repression complex SNAIL1-SMAD3/4.; Authors - Haiyu Wang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lana (Verified Customer) (07-15-2020)

SDS-PAGE: 20 ug/ul RIPA protein lysate. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1h, room T.Primary Ab: O/N incubation at 4C, 1:5000.Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1:15000.Lines of WB image: 1 – protein ladder, 2 - whole cell lysate, 3 – mitochondrial fraction lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse embryonic stem cells

SDHB Antibody Western Blot validation (1:5000 dilution) in Mouse embryonic stem cells (Cat no:10620-1-AP)