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SDHD Polyclonal antibody

SDHD Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26519-1-AP

Synonyms

PGL, CybS, CII-4, CII4, CII 4

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SDHD Polyclonal antibody (26519-1-AP)

    IHC staining of human stomach cancer using 26519-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26519-1-AP (SDHD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SDHD Polyclonal antibody (26519-1-AP)

    IF Staining of HepG2 using 26519-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SDHD antibody (26519-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26519-1-AP targets SDHD in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SDHD fusion protein Ag24644
Full Name succinate dehydrogenase complex, subunit D, integral membrane protein
Calculated Molecular Weight 17 kDa
GenBank Accession NumberBC012603
Gene Symbol SDHD
Gene ID (NCBI) 6392
RRIDAB_2880540
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for SDHD antibody 26519-1-APDownload protocol
IF protocol for SDHD antibody 26519-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Lana (Verified Customer) (09-07-2020)

Antibody detects multiple bands as well as specific 17kDa SDHD band on WB.SDS-PAGE: 15 ug/ul RIPA protein lysate, 4-12% Bis-Tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1h, room T.Primary Ab: O/N incubation at 4C, 1:1000.Secondary Ab: IRDye 680LT Goat anti-Rabbit, 1:15000.Lines of WB image: 1 – protein ladder, 2 and 3 – mitochondrial fraction lysates.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse embryonic stem cells
SDHD Antibody Western Blot validation (1:1000 dilution) in Mouse embryonic stem cells (Cat no:26519-1-AP)