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  • KD/KO Validated

SEC13 Polyclonal antibody

SEC13 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 15397-1-AP


D3S1231E, Protein SEC13 homolog, SEC13, SEC13 homolog (S. cerevisiae), SEC13 like protein 1, SEC13 related protein, SEC13L1, SEC13R

Tested Applications

Positive WB detected inHepG2 cells, RAW 264.7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse brain tissue, mouse liver tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human spleen tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15397-1-AP targets SEC13 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC13 fusion protein Ag7610
Full Name SEC13 homolog (S. cerevisiae)
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC002634
Gene Symbol SEC13
Gene ID (NCBI) 6396
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC13 is a component of the nuclear pore complex (NPC) and the COPII coat. It is required for esicle biogenesis from endoplasmic reticulum during the transport of proteins. Shuttling of SEC13 between the nucleus and the cytoplasm may couple and regulate functions between these two compartments. (PMID: 14517296; 16407955)


Product Specific Protocols
WB protocol for SEC13 antibody 15397-1-APDownload protocol
IHC protocol for SEC13 antibody 15397-1-APDownload protocol
IF protocol for SEC13 antibody 15397-1-APDownload protocol
IP protocol for SEC13 antibody 15397-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Structure of the nutrient-sensing hub GATOR2.

Authors - Max L Valenstein

Nat Commun

Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation

Authors - Xing Chen

Dev Cell

Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells

Authors - Wenxiu Dai

Redox Biol

Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis

Authors - Mengyue You

Proc Natl Acad Sci U S A

TFG facilitates outer coat disassembly on COPII transport carriers to promote tethering and fusion with ER-Golgi intermediate compartments.

Authors - Michael G Hanna

Proc Natl Acad Sci U S A

Atlastin-mediated membrane tethering is critical for cargo mobility and exit from the endoplasmic reticulum.

Authors - Liling Niu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Subir (Verified Customer) (09-27-2019)

clean and specific

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Protein from mouse tail tendon
SEC13 Antibody Western Blot, validation (1:500 dilution) in Protein from mouse tail tendon (Cat no:15397-1-AP)