|Positive WB detected in||HepG2 cells, RAW 264.7 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||mouse brain tissue, human lung tissue, mouse liver tissue, human ovary tissue, human testis tissue, human kidney tissue, human skin tissue, human spleen tissue, human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15397-1-AP targets SEC13 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SEC13 fusion protein Ag7610|
|Full Name||SEC13 homolog (S. cerevisiae)|
|Calculated molecular weight||36 kDa|
|Observed molecular weight||36 kDa|
|GenBank accession number||BC002634|
|Gene ID (NCBI)||6396|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SEC13 is a component of the nuclear pore complex (NPC) and the COPII coat. It is required for esicle biogenesis from endoplasmic reticulum during the transport of proteins. Shuttling of SEC13 between the nucleus and the cytoplasm may couple and regulate functions between these two compartments. (PMID: 14517296; 16407955)
|Product Specific Protocols|
|WB protocol for SEC13 antibody 15397-1-AP||Download protocol|
|IHC protocol for SEC13 antibody 15397-1-AP||Download protocol|
|IF protocol for SEC13 antibody 15397-1-AP||Download protocol|
|IP protocol for SEC13 antibody 15397-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Intracellular mannose binding lectin mediates subcellular trafficking of HIV-1 gp120 in neurons.
Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.
Proc Natl Acad Sci U S A
Atlastin-mediated membrane tethering is critical for cargo mobility and exit from the endoplasmic reticulum.
Proc Natl Acad Sci U S A
TFG facilitates outer coat disassembly on COPII transport carriers to promote tethering and fusion with ER-Golgi intermediate compartments.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Subir (Verified Customer) (09-27-2019)
clean and specific