SEC13 Polyclonal antibody

SEC13 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15397-1-AP

Synonyms

D3S1231E, Protein SEC13 homolog, SEC13, SEC13 homolog (S. cerevisiae), SEC13 like protein 1, SEC13 related protein, SEC13L1, SEC13R



Tested Applications

Positive WB detected inHepG2 cells, RAW 264.7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse brain tissue, human lung tissue, mouse liver tissue, human ovary tissue, human testis tissue, human kidney tissue, human skin tissue, human spleen tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

15397-1-AP targets SEC13 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC13 fusion protein Ag7610
Full Name SEC13 homolog (S. cerevisiae)
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC002634
Gene symbol SEC13
Gene ID (NCBI) 6396
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEC13 is a component of the nuclear pore complex (NPC) and the COPII coat. It is required for esicle biogenesis from endoplasmic reticulum during the transport of proteins. Shuttling of SEC13 between the nucleus and the cytoplasm may couple and regulate functions between these two compartments. (PMID: 14517296; 16407955)

Protocols

Product Specific Protocols
WB protocol for SEC13 antibody 15397-1-APDownload protocol
IHC protocol for SEC13 antibody 15397-1-APDownload protocol
IF protocol for SEC13 antibody 15397-1-APDownload protocol
IP protocol for SEC13 antibody 15397-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Neurobiol Dis

Intracellular mannose binding lectin mediates subcellular trafficking of HIV-1 gp120 in neurons.

Authors - C Teodorof
humanWB

Elife

Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.

Authors - Melissa Kane
humanWB

Proc Natl Acad Sci U S A

Atlastin-mediated membrane tethering is critical for cargo mobility and exit from the endoplasmic reticulum.

Authors - Liling Niu
ratWB

Proc Natl Acad Sci U S A

TFG facilitates outer coat disassembly on COPII transport carriers to promote tethering and fusion with ER-Golgi intermediate compartments.

Authors - Michael G Hanna

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Subir (Verified Customer) (09-27-2019)

clean and specific

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Protein from mouse tail tendon
SEC13 Antibody Western Blot, validation (1:500 dilution) in Protein from mouse tail tendon (Cat no:15397-1-AP)