SENP3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SENP3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 17659-1-AP

Synonyms

SUMO-1-specific protease 3, SSP3, SMT3IP1, Sentrin-specific protease 3, Sentrin/SUMO-specific protease SENP3

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Validation Data Gallery

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  WB analysis using 17659-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17659-1-AP (SENP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17659-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17659-1-AP (SENP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 17659-1-AP

  IP result of anti-SENP3 (IP:17659-1-AP, 4ug; Detection:17659-1-AP 1:500) with Y79 cells lysate 2000ug.

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  IHC staining of human ovary cancer using 17659-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17659-1-AP (SENP3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 17659-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17659-1-AP (SENP3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Y79 cells, rat embryo tissue
• Positive IP detected in: Y79 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

17659-1-AP targets SENP3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SENP3 fusion protein Ag11911
• Full Name: SUMO1/sentrin/SMT3 specific peptidase 3
• Calculated Molecular Weight: 574 aa, 65 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC080658
• Gene Symbol: SENP3
• Gene ID (NCBI): 26168
• RRID: AB_2301618
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SENP3(Sentrin-specific protease 3) is also named as SSP3, SUSP3 and belongs to the peptidase C48 family. It acts as an essential factor for ribosome biogenesis. SENP3 might regulate the sumoylation of other substrates through directly binding or mediating association with NPM1 at the nucleolus and other subcellular compartments.

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.; Authors - Fanis Pavlos P
human	WB,IF	FASEB J; SUMO1 SUMOylates and SENP3 deSUMOylates NLRP3 to orchestrate the inflammasome activation.; Authors - Luyao Shao
human	WB	Breast Cancer Res Treat; Targeting PELP1 oncogenic signaling in TNBC with the small molecule inhibitor SMIP34; Authors - Kristin A Altwegg
mouse	WB,IF	iScience; SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury; Authors - Qian Xia ; KD Validated
human	IP,WB	Mol Oncol; PELP1 inhibition by SMIP34 reduces endometrial cancer progression via attenuation of ribosomal biogenesis; Authors - Xue Yang
human,rat	WB	CNS Neurosci Ther; Astragaloside IV combined with ligustrazine ameliorates abnormal mitochondrial dynamics via Drp1 SUMO/deSUMOylation in cerebral ischemia-reperfusion injury; Authors - Xiangyu Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chun (Verified Customer) (07-03-2019)

This antibody is excellent.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293 and HeLa cells, and primary rat cortical neurons