SEPHS2 Polyclonal antibody

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  WB analysis using 14109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14109-1-AP (SEPHS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14109-1-AP (SEPHS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14109-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14109-1-AP (SEPHS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14109-1-AP (SEPHS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 14109-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14109-1-AP (SEPHS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SEPHS2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14109-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EC:2.7.9.3, Selenide, water dikinase 2, Selenium donor protein 2, Selenophosphate synthase 2, selenophosphate synthetase 2

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Tested Applications

• Positive WB detected in: NCI-H1299 cells, mouse pancreas tissue, mouse adipose tissue, rat liver tissue
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

14109-1-AP targets SEPHS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5255

  Product name: Recombinant human SEPHS2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 118-448 aa of BC016643

  Sequence: GMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECDNMLMLLSVSQSMSEEEREKVTPLMVKGFRDAAEEGGTAVTGGQTVVNPWIIIGGVATVVCQPNEFIMPDSAVVGDVLVLTKPLGTQVAVNAHQWLDNPERWNKVKMVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGILGHSQNLAKQQRNEVSFVIHNLPIIAKMAAVSKASGRFGLLQGTSAETSGGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGATAAVLAPDSSNASSEPSS

• Full Name: selenophosphate synthetase 2
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC016643
• Gene Symbol: SEPHS2
• Gene ID (NCBI): 22928
• RRID: AB_2878014
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99611
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEPHS2 (Selenophosphate synthetase 2), a selenophosphate synthetase, catalyzes the conversion of selenite and adenosine triphosphate (ATP) into selenophosphate. SEPHS2 is the rate-limiting enzyme that converts selenite to selenocysteine, a key substance in the synthesis of many selenium proteins such as glutathione peroxidase, thioredoxin reductase, and formate dehydrogenase (PMID: 40750759, 31695102).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; PRDX6 dictates ferroptosis sensitivity by directing cellular selenium utilization; Authors - Junya Ito
	WB	Biomaterials; H2Se-evolving bio-heterojunctions promote cutaneous regeneration in infected wounds by inhibiting excessive cellular senescence; Authors - Fan Yang
human	WB	EMBO Mol Med; Breast cancer secretes anti-ferroptotic MUFAs and depends on selenoprotein synthesis for metastasis; Authors - Tobias Ackermann
human	IHC	Front Med (Lausanne); A glycometabolic gene signature associating with immune infiltration and chemosensitivity and predicting the prognosis of patients with osteosarcoma; Authors - Fengyan Wang
mouse,human	WB	Nat Struct Mol Biol; PRDX6 augments selenium utilization to limit iron toxicity and ferroptosis; Authors - Hiroaki Fujita
human	WB	Cell Death Dis; METTL5 regulates SEPHS2-mediated selenoprotein synthesis to promote multiple myeloma survival and progression; Authors - Junyao Jiang