SEPHS2 Polyclonal antibody

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SEPHS2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14109-1-AP Copied!

Publications (12)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

EC:2.7.9.3, Selenide, water dikinase 2, Selenium donor protein 2, Selenophosphate synthase 2, selenophosphate synthetase 2

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  WB analysis using 14109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14109-1-AP (SEPHS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 14109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14109-1-AP (SEPHS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 14109-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14109-1-AP (SEPHS2 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 14109-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14109-1-AP (SEPHS2 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 14109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14109-1-AP (SEPHS2 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 14109-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14109-1-AP (SEPHS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 14109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14109-1-AP (SEPHS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 14109-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14109-1-AP (SEPHS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: NCI-H1299 cells, mouse pancreas tissue, mouse adipose tissue, rat liver tissue
• Positive IHC detected in: mouse pancreas tissue, human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14109-1-AP targets SEPHS2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5255

  Product name: Recombinant human SEPHS2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 118-448 aa of BC016643

  Sequence: GMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECDNMLMLLSVSQSMSEEEREKVTPLMVKGFRDAAEEGGTAVTGGQTVVNPWIIIGGVATVVCQPNEFIMPDSAVVGDVLVLTKPLGTQVAVNAHQWLDNPERWNKVKMVVSREEVELAYQEAMFNMATLNRTAAGLMHTFNAHAATDITGFGILGHSQNLAKQQRNEVSFVIHNLPIIAKMAAVSKASGRFGLLQGTSAETSGGLLICLPREQAARFCSEIKSSKYGEGHQAWIVGIVEKGNRTARIIDKPRVIEVLPRGATAAVLAPDSSNASSEPSS

• Full Name: selenophosphate synthetase 2
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC016643
• Gene Symbol: SEPHS2
• Gene ID (NCBI): 22928
• RRID: AB_2878014
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99611
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEPHS2 (Selenophosphate synthetase 2), a selenophosphate synthetase, catalyzes the conversion of selenite and adenosine triphosphate (ATP) into selenophosphate. SEPHS2 is the rate-limiting enzyme that converts selenite to selenocysteine, a key substance in the synthesis of many selenium proteins such as glutathione peroxidase, thioredoxin reductase, and formate dehydrogenase (PMID: 40750759, 31695102).

Publications

What published studies show

SEPHS2 Polyclonal antibody (Cat# 14109-1-AP) has 12 citations published in journals including Molecular Cell, Nature Structural & Molecular Biology, Redox Biology, Biomaterials, Cell Death & Disease, EMBO Molecular Medicine, Cell Reports, Journal of Nanobiotechnology, Scientific Reports, Journal of Dairy Science, and Frontiers in Medicine. Associated research fields span ferroptosis, selenoprotein synthesis, cancer biology (hepatocellular carcinoma, breast cancer, multiple myeloma, pancreatic cancer, osteosarcoma), wound healing, atherosclerosis, and mastitis.

Automatically generated from the 12 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Redox Biol; Macrophages and macrophage extracellular vesicles confer cancer ferroptosis resistance via PRDX6-mediated mitophagy inhibition; Authors - Naisheng Zheng
human	WB	Mol Cell; PRDX6 dictates ferroptosis sensitivity by directing cellular selenium utilization; Authors - Junya Ito
		Mol Cell; Hypoxia-driven lncRNA SHIELD promotes GPX4 translation and protects against ferroptosis in hepatocellular carcinoma.; Authors - Kejia Liu
mouse	WB	J Nanobiotechnology; Targeting VSMCs to suppress macrophage-like phenotype switching that restrains atherogenesis by immunosuppressive oligodeoxynucleotide (A151) functionalized selenium nanoparticles.; Authors - Jingru Wang
	WB	Biomaterials; H2Se-evolving bio-heterojunctions promote cutaneous regeneration in infected wounds by inhibiting excessive cellular senescence; Authors - Fan Yang
human	WB	Cell Death Dis; METTL5 regulates SEPHS2-mediated selenoprotein synthesis to promote multiple myeloma survival and progression; Authors - Junyao Jiang