SERPINE2 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82732-5-RR (same clone as 82732-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82732-5-RR (SERPINE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82732-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 82732-5-RR (same clone as 82732-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82732-5-RR (SERPINE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82732-5-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 82732-5-RR (same clone as 82732-5-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug SERPINE2 Recombinant antibody (82732-5-RR, Clone:5O16) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82732-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82732-5-RR

  Biolayer interferometry (BLl) kinetic assays of 82732-5-RR against Human SERPINE2 were performed. The affinity constant is below 1 pM.

SERPINE2 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 82732-5-PBS

Clone No.5O16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

PI7, PN1, Serpin E2, 5O16, GDN

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Product Information

82732-5-PBS targets SERPINE2 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1830

  Product name: Recombinant human SERPINE2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 28-397 aa of BC015663

  Sequence: EELGSNTGIQVFNQIVKSRPHDNIVISPHGIASVLGMLQLGADGRTKKQLAMVMRYGVNGVGKILKKINKAIVSKKNKDIVTVANAVFVKNASEIEVPFVTRNKDVFQCEVRNVNFEDPASACDSINAWVKNETRDMIDNLLSPDLIDGVLTRLVLVNAVYFKGLWKSRFQPENTKKRTFVAADGKSYQVPMLAQLSVFRCGSTSAPNDLWYNFIELPYHGESISMLIALPTESSTPLSAIIPHISTKTIDSWMSIMVPKRVQVILPKFTAVAQTDLKEPLKVLGITDMFDSSKANFAKITTGSENLHVSHILQKAKIEVSEDGTKASAATTAILIARSSPPWFIVDRPFLFFIRHNPTGAVLFMGQINK

• Full Name: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44-50 kDa
• GenBank Accession Number: BC015663
• Gene Symbol: SERPINE2
• Gene ID (NCBI): 5270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07093
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.