Neuroserpin Monoclonal antibody

Neuroserpin Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human, pig, rat and More (1)







Cat no : 66997-1-Ig


DKFZp781N13156, neuroserpin, Peptidase inhibitor 12, PI 12, PI12, Serpin I1, SERPINI1

Tested Applications

Positive WB detected inpig brain tissue
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU-251 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66997-1-Ig targets Neuroserpin in WB, IF, IHC, ELISA applications and shows reactivity with Human, pig, rat samples.

Tested Reactivity Human, pig, rat
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Neuroserpin fusion protein Ag28636
Full Name serpin peptidase inhibitor, clade I (neuroserpin), member 1
Calculated Molecular Weight 410 aa, 47 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC018043
Gene Symbol SERPINI1
Gene ID (NCBI) 5274
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.


Product Specific Protocols
WB protocol for Neuroserpin antibody 66997-1-IgDownload protocol
IHC protocol for Neuroserpin antibody 66997-1-IgDownload protocol
IF protocol for Neuroserpin antibody 66997-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Brain Pathol

Serpin neuropathology in the P497S UBQLN2 mouse model of ALS/FTD.

Authors - Nicole R Higgins