SETD7 Monoclonal antibody

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• SETD7 Monoclonal antibody (68569-1-Ig)

SETD7 Monoclonal Antibody for WB, ELISA

Cat No. 68569-1-Ig

Clone No.1C1A9

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, ELISA

Synonyms

FLJ21193, H3 K4 HMTase SETD7, KIAA1717, KMT7, Lysine N methyltransferase 7, SET7, SET7/9, SET9, SETD7

• 

  WB analysis using 68569-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68569-1-Ig (SETD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 68569-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68569-1-Ig (SETD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: LNCaP cells, Jurkat cells, MCF-7 cells, HeLa cells, HEK-293 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68569-1-Ig targets SETD7 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33803

  Product name: Recombinant human SETD7 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-366 aa of BC121055

  Sequence: MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK

• Full Name: SET domain containing (lysine methyltransferase) 7
• Calculated Molecular Weight: 366 aa, 41 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC121055
• Gene Symbol: SETD7
• Gene ID (NCBI): 80854
• RRID: AB_3085271
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q8WTS6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SET domain containing lysine methyltransferase 7 (SETD7, also called SET7/9) was the first lysine methyltransferase (KMT) discovered to specifically monomethylate lysine-4 of histone 3 (H3K4me1) (PMID: 26435321). SETD7 has been shown to have a very broad target specificity in vitro, including transcriptional regulators such as TAF10, p53, ER, p65, STAT3, Rb, Mypt, Tat, and Foxo3 (PMID: 25136132, 30013796).

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human, mouse	WB	Cell Signal; Set7 regulates ferroptosis in pulmonary arterial hypertension endothelial cells through the DPP4/NOX4 axis mediated by H3K4me1 modification.; Authors - Jingyuan Chen