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SETD7 Monoclonal antibody, PBS Only (Capture) (68569-1-PBS)

SETD7 Monoclonal antibody, PBS Only (Capture)

Name: SETD7 Monoclonal antibody, PBS Only (Capture), 100ug
Brand: Proteintech
SKU: 68569-1-PBS-100UG

SETD7 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 68569-1-PBS

Clone No. 1C1A9

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Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

1C1A9, EC:2.1.1.364, H3 K4 HMTase SETD7, H3-K4-HMTase SETD7, Histone H3-K4 methyltransferase SETD7

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllWBCytometric bead array sample testCytometric bead array

WB analysis using 68569-1-Ig (same clone as 68569-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68569-1-Ig (SETD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68569-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Various lysates were subjected to SDS PAGE followed by western blot with 68569-1-Ig (SETD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68569-1-PBS in a different storage buffer formulation.
WB analysis using 68569-1-Ig (same clone as 68569-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68569-1-Ig (SETD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68569-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Various lysates were subjected to SDS PAGE followed by western blot with 68569-1-Ig (SETD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68569-1-PBS in a different storage buffer formulation.
Cytometric bead array sample test of MP51900-1
Cytometric bead array sample test of MP51900-1,SETD7 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68569-1-PBS. Detection antibody: 68569-2-PBS.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array standard curve of MP51900-1
Cytometric bead array standard curve of MP51900-1, SETD7 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68569-1-PBS. Detection antibody: 68569-2-PBS. Standard:Ag33803. Range: 0.781-100 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
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MSDS SDS

Product Information

68569-1-PBS targets SETD7 as part of a matched antibody pair:

MP51900-1: 68569-1-PBS capture and 68569-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag33803 Product name: Recombinant human SETD7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-366 aa of BC121055 Sequence: MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK Predict reactive species
Full Name	SET domain containing (lysine methyltransferase) 7
Calculated Molecular Weight	366 aa, 41 kDa
Observed Molecular Weight	48-50 kDa
GenBank Accession Number	BC121055
Gene Symbol	SETD7
Gene ID (NCBI)	80854
RRID	AB_3085271
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q8WTS6
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SET domain containing lysine methyltransferase 7 (SETD7, also called SET7/9) was the first lysine methyltransferase (KMT) discovered to specifically monomethylate lysine-4 of histone 3 (H3K4me1) (PMID: 26435321). SETD7 has been shown to have a very broad target specificity in vitro, including transcriptional regulators such as TAF10, p53, ER, p65, STAT3, Rb, Mypt, Tat, and Foxo3 (PMID: 25136132, 30013796).