SFRP2 Monoclonal antibody, PBS Only (Detector)

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  WB analysis using 66328-1-Ig (same clone as 66328-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66328-1-Ig (same clone as 66328-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 66328-1-Ig (same clone as 66328-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66328-1-Ig (same clone as 66328-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66328-1-Ig (SFRP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66328-1-Ig (SFRP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66328-1-Ig (same clone as 66328-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SFRP2 antibody (66328-1-Ig, Clone: 1F3A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51471-1

  Cytometric bead array sample test of MP51471-1, SFRP2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66328-2-PBS. Detection antibody: 66328-1-PBS.

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  Cytometric bead array standard curve of MP51471-1

  Cytometric bead array standard curve of MP51471-1, SFRP2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66328-2-PBS. Detection antibody: 66328-1-PBS. Standard:Ag18840. Range: 0.781-100 ng/mL.

SFRP2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 66328-1-PBS

Clone No.1F3A1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

1F3A1, FKSG12, SARP1, SDF-5, Secreted apoptosis-related protein 1

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Product Information

66328-1-PBS targets SFRP2 as part of a matched antibody pair:

MP51471-1: 66328-2-PBS capture and 66328-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18840

  Product name: Recombinant human SFRP2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-295 aa of BC008666

  Sequence: MLQGPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPVNLQLCHGIEYQNMRLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRFPQDNDLCIPLASSDHLLPATEEAPKVCEACKNKNDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKKSVLWLKDSLQCTCEEMNDINAPYLVMGQKQGGELVITSVKRWQKGQREFKRISRSIRKLQC

• Full Name: secreted frizzled-related protein 2
• Calculated Molecular Weight: 295 aa, 34 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC008666
• Gene Symbol: SFRP2
• Gene ID (NCBI): 6423
• RRID: AB_2881709
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96HF1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.