Tested Applications
| Positive WB detected in | pig brain tissue, Y79 cells, COLO 320 cells, rat brain tissue, rabbit brain tissue, mouse brain tissue |
| Positive IHC detected in | human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66328-1-Ig targets SFRP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18840 Product name: Recombinant human SFRP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-295 aa of BC008666 Sequence: MLQGPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPVNLQLCHGIEYQNMRLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRFPQDNDLCIPLASSDHLLPATEEAPKVCEACKNKNDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKKSVLWLKDSLQCTCEEMNDINAPYLVMGQKQGGELVITSVKRWQKGQREFKRISRSIRKLQC Predict reactive species |
| Full Name | secreted frizzled-related protein 2 |
| Calculated Molecular Weight | 295 aa, 34 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC008666 |
| Gene Symbol | SFRP2 |
| Gene ID (NCBI) | 6423 |
| RRID | AB_2881709 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96HF1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SFRP2 antibody 66328-1-Ig | Download protocol |
| IHC protocol for SFRP2 antibody 66328-1-Ig | Download protocol |
| WB protocol for SFRP2 antibody 66328-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SFRP2 Monoclonal antibody (1F3A1), catalog number 66328-1-Ig, has 8 citations published in Autophagy, Nature Communications, Journal of Pharmaceutical Analysis, Journal of Translational Medicine, Frontiers in Immunology, International Journal of Molecular Sciences, Journal of Translational Autoimmunity, and Experimental Cell Research. Research fields span pulmonary fibrosis and mitophagy, uterine scarring, antitumor immunotherapy, ovarian cancer metabolism, liver metastasis, tooth mineralization, systemic sclerosis, and hepatocellular carcinoma.
| Species | Application | Title |
|---|---|---|
Autophagy Autocrine SFRP2 (secreted frizzled related protein 2) enhances lung myofibroblast fibrogenic activity by suppressing PINK1-mediated mitophagy initiation. | ||
Nat Commun Identifying fibroblast-derived sFRP2 as a therapeutic target and engineering siRNA therapy for uterine scarring
| ||
J Pharm Anal A novel proteolysis-targeting chimera strategy targeting multiple immune checkpoints containing ITIMs enhances antitumor immunity. | ||
J Transl Med SFRP2 drives aerobic glycolysis and tumor progression in ovarian cancer by transcriptional upregulation of PTK2B. | ||
Front Immunol Integrated single-cell RNA sequencing reveals the tumor heterogeneity and microenvironment landscape during liver metastasis in adenocarcinoma of esophagogastric junction | ||
Int J Mol Sci MFAP5 Activates ITGA5 to Drive Tooth Germ Mineralization Through the MAPK/ERK Pathway: Insights from Single-Cell Transcriptomics. |
Reviews
What customers say
Both reviewers rated this antibody 5 stars. One user found it performed well for Western Blot and Immunofluorescence at 1:3000 on iPS-derived cardiomyocytes and fibroblasts, noting strong fluorescence signals in confocal imaging at 5–10% intensity. The other reported good results for mouse liver tissue IF at 1:200 with overnight incubation. Overall, users report reliable performance across applications with clear signals.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Asim (Verified Customer) (12-12-2025) | This antibody worked well with both WB and IF at 1:3000. Fluorescence signals were strong in confocal imaging at 5-10% intensity.
|
Kenzo (Verified Customer) (08-30-2023) | This antibody worked well for mouse tissues when used at 1:200 and incubated for overnight.
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