Bif-1 Polyclonal antibody

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  WB analysis of mouse kidney using 15422-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15422-1-AP

  WB result of Bif-1 antibody (15422-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Bif-1 transfected HepG2 cells.

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  WB analysis of mouse heart using 15422-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 15422-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15422-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 15422-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15422-1-AP

  IP result of anti-Bif-1 (IP:15422-1-AP, 4ug; Detection:15422-1-AP 1:400) with HEK-293 cells lysate 2000 ug.

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  IHC staining of mouse kidney using 15422-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15422-1-AP (Bif-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 15422-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15422-1-AP (Bif-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15422-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Bif-1 antibody (15422-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

Bif-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15422-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

SH3GLB1, Bax interacting factor 1, Bax-interacting factor 1, Bif1, Endophilin B1

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells, HepG2 cells, human placenta tissue, mouse heart tissue, mouse placenta tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: mouse kidney tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Product Information

15422-1-AP targets Bif-1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7710

  Product name: Recombinant human SH3GLB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-365 aa of BC007455

  Sequence: MNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKIWTEKIMKQTEVLLQPNPNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIKCGETQKRIGTADRELIQTSALNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLKKAKAAETRNSSEQELRITQSEFDRQAEITRLLLEGISSTHAHHLRCLNDFVEAQMTYYAQCYQYMLDLQKQLGSFPSNYLSNNNQTSVTPVPSVLPNAIGSSAMASTSGLVITSPSNLSDLKECSGSRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERGNQKGKVPITYLELLN

• Full Name: SH3-domain GRB2-like endophilin B1
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC007455
• Gene Symbol: Bif-1
• Gene ID (NCBI): 51100
• RRID: AB_2254564
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y371
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SH3GLB1 also known as Bif-1, contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain, but shares no significant homology with members of the Bcl-2 family. SH3GLB1 is involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Biomed Mater; Nano implant surface triggers autophagy through membrane curvature distortion to regulate the osteogenic differentiation; Authors - Guangwen Li
	WB	Mediators Inflamm; Sodium Houttuyfonate Ameliorates β-amyloid1-42-Induced Memory Impairment and Neuroinflammation through Inhibiting the NLRP3/GSDMD Pathway in Alzheimer's Disease.; Authors - Yuequan Zhao
rat	WB,IHC	Eur J Pharmacol; SUMO2-mediated SUMOylation of SH3GLB1 promotes ionizing radiation-induced hypertrophic cardiomyopathy through mitophagy activation.; Authors - Anbo Gao
human	WB	J Exp Clin Cancer Res; SH3GLB1-related autophagy mediates mitochondrial metabolism to acquire resistance against temozolomide in glioblastoma.; Authors - Chia-Hung Chien
mouse	WB	Oncotarget; Autophagy-related gene expression is an independent prognostic indicator of glioma.; Authors - Huixue Zhang
human	WB,ChIP	BMC Cancer; Role of SH3GLB1 in the regulation of CD133 expression in GBM cells; Authors - Chia-Hung Chien