Bif-1 Polyclonal antibody
Bif-1 Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 15422-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse kidney tissue, HEK-293 cells, human placenta tissue, mouse heart tissue, mouse placenta tissue |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 4 publications below |
IHC | See 1 publications below |
ChIP | See 1 publications below |
Product Information
15422-1-AP targets Bif-1 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Bif-1 fusion protein Ag7710 |
Full Name | SH3-domain GRB2-like endophilin B1 |
Calculated molecular weight | 41 kDa |
Observed molecular weight | 41 kDa |
GenBank accession number | BC007455 |
Gene symbol | SH3GLB1 |
Gene ID (NCBI) | 51100 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for Bif-1 antibody 15422-1-AP | Download protocol |
IHC protocol for Bif-1 antibody 15422-1-AP | Download protocol |
IF protocol for Bif-1 antibody 15422-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Eur J Pharmacol SUMO2-mediated SUMOylation of SH3GLB1 promotes ionizing radiation-induced hypertrophic cardiomyopathy through mitophagy activation. | ||
J Exp Clin Cancer Res SH3GLB1-related autophagy mediates mitochondrial metabolism to acquire resistance against temozolomide in glioblastoma. | ||
Oncotarget Autophagy-related gene expression is an independent prognostic indicator of glioma. | ||