Bif-1 Polyclonal antibody

Bif-1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15422-1-AP

Synonyms

Bax interacting factor 1, Bif 1, Bif1, Endophilin B1, SH3GLB1



Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells, human placenta tissue, mouse heart tissue, mouse placenta tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

15422-1-AP targets Bif-1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Bif-1 fusion protein Ag7710
Full Name SH3-domain GRB2-like endophilin B1
Calculated molecular weight 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC007455
Gene symbol SH3GLB1
Gene ID (NCBI) 51100
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Bif-1 antibody 15422-1-APDownload protocol
IHC protocol for Bif-1 antibody 15422-1-APDownload protocol
IF protocol for Bif-1 antibody 15422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Oncotarget

Autophagy-related gene expression is an independent prognostic indicator of glioma.

Authors - Huixue Zhang