Validation Data Gallery
|Positive WB detected in||mouse kidney tissue, HEK-293 cells, human placenta tissue, mouse heart tissue, mouse placenta tissue|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15422-1-AP targets Bif-1 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Bif-1 fusion protein Ag7710|
|Full Name||SH3-domain GRB2-like endophilin B1|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||41 kDa|
|GenBank accession number||BC007455|
|Gene ID (NCBI)||51100|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
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