Bif-1 Polyclonal antibody

Bif-1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15422-1-AP

Synonyms

Bax interacting factor 1, Bif 1, Bif1, Endophilin B1, SH3GLB1

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Validation Data Gallery

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  WB analysis of mouse kidney using 15422-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15422-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15422-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15422-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15422-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15422-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15422-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15422-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 15422-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 15422-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15422-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 15422-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 15422-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15422-1-AP (Bif-1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15422-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15422-1-AP (Bif-1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 15422-1-AP

  Immunofluorescent analysis of HepG2 cells, using SH3GLB1 antibody 15422-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells, human placenta tissue, mouse heart tissue, mouse placenta tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Product Information

15422-1-AP targets Bif-1 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Bif-1 fusion protein Ag7710
• Full Name: SH3-domain GRB2-like endophilin B1
• Calculated molecular weight: 41 kDa
• Observed molecular weight: 41 kDa
• GenBank accession number: BC007455
• Gene symbol: SH3GLB1
• Gene ID (NCBI): 51100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB,IHC	Eur J Pharmacol; SUMO2-mediated SUMOylation of SH3GLB1 promotes ionizing radiation-induced hypertrophic cardiomyopathy through mitophagy activation.; Authors - Anbo Gao
human	WB	J Exp Clin Cancer Res; SH3GLB1-related autophagy mediates mitochondrial metabolism to acquire resistance against temozolomide in glioblastoma.; Authors - Chia-Hung Chien
mouse	WB	Oncotarget; Autophagy-related gene expression is an independent prognostic indicator of glioma.; Authors - Huixue Zhang
human	WB,ChIP	BMC Cancer; Role of SH3GLB1 in the regulation of CD133 expression in GBM cells; Authors - Chia-Hung Chien