SHE Polyclonal antibody

SHE Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22902-1-AP

Synonyms

RP11 350G8.8, SHE

---

Validation Data Gallery

• 

  WB analysis of mouse testis using 22902-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22902-1-AP (SHE Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22902-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22902-1-AP (SHE Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 22902-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22902-1-AP (SHE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22902-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22902-1-AP (SHE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 22902-1-AP

  Immunofluorescent analysis of HepG2 cells using 22902-1-AP (SHE antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22902-1-AP targets SHE in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Src homology 2 domain containing E
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: NM_001010846
• Gene Symbol: SHE
• Gene ID (NCBI): 126669
• RRID: AB_2879185
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SHE, also known as SH2 domain-containing adapter protein E, is a 495 amino acid protein whose molecular weight is 54 kDa. The function of SHE remains unknown. This antibody specifically recognises the 54 kDa protein.