DSS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DSS1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 13639-1-AP

Synonyms

DSS1, ECD, SEM1, SHFD1, Shfdg1, SHFM1, SHSF1

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Validation Data Gallery

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  IHC staining of human skin cancer using 13639-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13639-1-AP (DSS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 13639-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13639-1-AP (DSS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13639-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13639-1-AP (DSS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13639-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13639-1-AP (DSS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 13639-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13639-1-AP (DSS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 13639-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13639-1-AP (DSS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human skin cancer tissue, human cervical cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

13639-1-AP targets DSS1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DSS1 fusion protein Ag4573
• Full Name: split hand/foot malformation (ectrodactyly) type 1
• Calculated Molecular Weight: 70 aa, 8 kDa
• GenBank Accession Number: BC032782
• Gene Symbol: SHFM1
• Gene ID (NCBI): 7979
• RRID: AB_2254633
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.; Authors - Vaibhav Bhatia ; KD Validated
human	WB, IF	EMBO J; The proteasomal de-ubiquitinating enzyme POH1 promotes the double-strand DNA break response.; Authors - Laura R Butler
		J Med Genet; Absent expression of the osteoblast-specific maternally imprinted genes, DLX5 and DLX6, causes split hand/split foot malformation type I.; Authors - Sawitree Rattanasopha
human	IHC	Lab Invest; Increased chemosensitivity via BRCA2-independent DNA damage in DSS1- and PCID2-depleted breast carcinomas.; Authors - Naomi Gondo ; KD Validated
human	WB	BMC Cancer; Breast cancers with high DSS1 expression that potentially maintains BRCA2 stability have poor prognosis in the relapse-free survival.; Authors - Andri Rezano
human	WB	Protein Cell; DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells.; Authors - Yinghao Zhang