SHMT1 Polyclonal antibody

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  WB analysis of HeLa using 14149-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14149-1-AP (SHMT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14149-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14149-1-AP (SHMT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14149-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14149-1-AP (SHMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14149-1-AP

  Immunofluorescent analysis of HepG2 cells using 14149-1-AP (SHMT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

SHMT1 Polyclonal Antibody for WB, IF, IHC, ELISA

Cat No. 14149-1-AP

Publications(12)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CSHMT, Serine methylase, SHMT, SHMT1

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 2 publications below

Product Information

14149-1-AP targets SHMT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5330

  Product name: Recombinant human SHMT1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 134-483 aa of BC038598

  Sequence: PHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGYINYDQLEENARLFHPKLIIAGTSCYSRNLEYARLRKIADENGAYLMADMAHISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCRAGMIFYRKGVKSVDPKTGKEILYNLESLINSAVFPGLQGGPHNHAIAGVAVALKQAMTLEFKVYQHQVVANCRALSEALTELGYKIVTGGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASLFPLPGLPDF

• Full Name: serine hydroxymethyltransferase 1 (soluble)
• Calculated Molecular Weight: 483 aa, 53 kDa
• Observed Molecular Weight: 45-53 kDa
• GenBank Accession Number: BC038598
• Gene Symbol: SHMT1
• Gene ID (NCBI): 6470
• RRID: AB_2239222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P34896
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SHMT1 is the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. This antibody can detect both 49 kDa and 53 kDa isoforms in western blotting.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
mouse	WB	Cell Metab; PMID: 28111214; Authors - Eric H Ma
human	WB	Nat Commun; Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.; Authors - Eduardo Balsa
human	WB	PLoS Pathog; SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.; Authors - Muyu Xu ; KD Validated
human	WB	Sci Rep; Vascular endothelial growth factor signaling requires glycine to promote angiogenesis.; Authors - Dongqing Guo
human	IF	J Cell Sci; Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway.; Authors - S John Calise

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (03-26-2021)

Good Antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse liver, mouse brain / Hella, HepG2

SHMT1 Antibody Western Blot validation (1:2000 dilution) in mouse liver, mouse brain / Hella, HepG2 (Cat no:14149-1-AP)