|Positive WB detected in||HeLa cells, HepG2 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
14149-1-AP targets SHMT1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SHMT1 fusion protein Ag5330|
|Full Name||serine hydroxymethyltransferase 1 (soluble)|
|Calculated molecular weight||483 aa, 53 kDa|
|Observed molecular weight||45-49, 53 kDa|
|GenBank accession number||BC038598|
|Gene ID (NCBI)||6470|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SHMT1 is the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. This antibody can detect both 49 kDa and 53 kDa isoforms in western blotting.
Proteomic and metabolomic analyses uncover sex-specific regulatory pathways in mouse fetal germline differentiation.
J Cell Sci
Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway.
Vascular endothelial growth factor signaling requires glycine to promote angiogenesis.
SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.
Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.
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Hua (Verified Customer) (03-26-2021)