• Featured Product
  • KD/KO Validated

SHMT1 Polyclonal antibody

SHMT1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14149-1-AP

Synonyms

CSHMT, Serine methylase, SHMT, SHMT1



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14149-1-AP targets SHMT1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHMT1 fusion protein Ag5330
Full Name serine hydroxymethyltransferase 1 (soluble)
Calculated molecular weight 483 aa, 53 kDa
Observed molecular weight 45-49, 53 kDa
GenBank accession numberBC038598
Gene symbol SHMT1
Gene ID (NCBI) 6470
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SHMT1 is the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. This antibody can detect both 49 kDa and 53 kDa isoforms in western blotting.

Protocols

Product Specific Protocols
WB protocol for SHMT1 antibody 14149-1-APDownload protocol
IHC protocol for SHMT1 antibody 14149-1-APDownload protocol
IF protocol for SHMT1 antibody 14149-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Biol Reprod

Proteomic and metabolomic analyses uncover sex-specific regulatory pathways in mouse fetal germline differentiation.

Authors - Yohei Hayashi
humanIF

J Cell Sci

Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway.

Authors - S John Calise
humanWB

Sci Rep

Vascular endothelial growth factor signaling requires glycine to promote angiogenesis.

Authors - Dongqing Guo
humanWB

PLoS Pathog

SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.

Authors - Muyu Xu
  • KD Validated
humanWB

Nat Commun

Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.

Authors - Eduardo Balsa
mouseWB

Cell Metab

PMID: 28111214

Authors - Eric H Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hua (Verified Customer) (03-26-2021)

Good Antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse liver, mouse brain / Hella, HepG2
SHMT1 Antibody Western Blot validation (1:2000 dilution) in mouse liver, mouse brain / Hella, HepG2 (Cat no:14149-1-AP)