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SIRT1 Monoclonal antibody
SIRT1 Monoclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 60303-1-Ig
|Positive WB detected in||HeLa cells, HEK-293 cells, Jurkat cells, MCF-7 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, mouse testis tissue, human colon tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
60303-1-Ig targets SIRT1 in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||SIRT1 fusion protein Ag17677|
|Full Name||sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)|
|Calculated molecular weight||747 aa, 82 kDa|
|Observed molecular weight||110-130 kDa|
|GenBank accession number||BC012499|
|Gene ID (NCBI)||23411|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SIRT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SIRT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. It can shuttles between nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.
|Product Specific Protocols|
|WB protocol for SIRT1 antibody 60303-1-Ig||Download protocol|
|IHC protocol for SIRT1 antibody 60303-1-Ig||Download protocol|
|IF protocol for SIRT1 antibody 60303-1-Ig||Download protocol|
|IP protocol for SIRT1 antibody 60303-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Catalytic-independent neuroprotection by SIRT1 is mediated through interaction with HDAC1.
Microglia Activated by Excess Cortisol Induce HMGB1 Acetylation and Neuroinflammation in the Hippocampal DG Region of Mice Following Cold Exposure.
Int J Chron Obstruct Pulmon Dis
SIRT1 protects against urban particulate matter-induced airway inflammation.
Exp Ther Med
TP53 mutation influences the efficacy of treatment of colorectal cancer cell lines with a combination of sirtuin inhibitors and chemotherapeutic agents.
Mol Med Rep
Ameliorative effects and mechanism of crocetin in arsenic trioxide‑induced cardiotoxicity in rats.
J Mol Neurosci
Endurance Exercise-Induced Autophagy/Mitophagy Coincides with a Reinforced Anabolic State and Increased Mitochondrial Turnover in the Cortex of Young Male Mouse Brain.