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SIRT1 Monoclonal antibody

SIRT1 Monoclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

7F6G6

Cat no : 60303-1-Ig

Synonyms

hSIR2, hSIRT1, SIR2 like protein 1, SIR2L1, SIRT1



Tested Applications

Positive WB detected inHeLa cells, K-562 cells, Hek-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, mouse testis tissue, human colon tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60303-1-Ig targets SIRT1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SIRT1 fusion protein Ag17677
Full Name sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
Calculated molecular weight 747 aa, 82 kDa
Observed molecular weight 110-130 kDa
GenBank accession numberBC012499
Gene symbol SIRT1
Gene ID (NCBI) 23411
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. It can shuttles between nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.

Protocols

Product Specific Protocols
WB protocol for SIRT1 antibody 60303-1-IgDownload protocol
IHC protocol for SIRT1 antibody 60303-1-IgDownload protocol
IF protocol for SIRT1 antibody 60303-1-IgDownload protocol
IP protocol for SIRT1 antibody 60303-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

PLoS One

Catalytic-independent neuroprotection by SIRT1 is mediated through interaction with HDAC1.

Authors - Jason A Pfister
mouseWB

Biomolecules

Microglia Activated by Excess Cortisol Induce HMGB1 Acetylation and Neuroinflammation in the Hippocampal DG Region of Mice Following Cold Exposure.

Authors - Bin Xu
humanIHC

Int J Chron Obstruct Pulmon Dis

SIRT1 protects against urban particulate matter-induced airway inflammation.

Authors - Tianwen Lai
humanWB

Exp Ther Med

TP53 mutation influences the efficacy of treatment of colorectal cancer cell lines with a combination of sirtuin inhibitors and chemotherapeutic agents.

Authors - Hao Yang
ratWB

Mol Med Rep

Ameliorative effects and mechanism of crocetin in arsenic trioxide‑induced cardiotoxicity in rats.

Authors - Zhifeng Zhao
mouseWB

J Mol Neurosci

Endurance Exercise-Induced Autophagy/Mitophagy Coincides with a Reinforced Anabolic State and Increased Mitochondrial Turnover in the Cortex of Young Male Mouse Brain.

Authors - Insu Kwon