|Positive WB detected in||HeLa cells, NCCIT cell, PC-3 cells, L02 cells|
|Western Blot (WB)||WB : 1:20000-1:80000|
|Sample-dependent, check data in validation data gallery|
66543-1-Ig targets SIRT4 in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
|Tested Reactivity||Human, mouse, rat, pig|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||SIRT4 fusion protein Ag17347|
|Full Name||sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)|
|Calculated molecular weight||314 aa, 35 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||BC109319|
|Gene ID (NCBI)||23409|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development.
miR-424-5p regulates cell proliferation and migration of esophageal squamous cell carcinoma by targeting SIRT4.
Int J Biochem Cell Biol
SIRT4 regulates rat dental papilla cell differentiation by promoting mitochondrial functions.
SIRT4 is the molecular switch mediating cellular proliferation in colorectal cancer through GLS mediated activation of AKT/GSK3β/CyclinD1 pathway.
Dis Model Mech
Ammonia inhibits energy metabolism in astrocytes in a rapid and glutamate dehydrogenase 2-dependent manner.
20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1α pathway: A translational study.