|Positive WB detected in||HeLa cells, NCCIT cell, PC-3 cells, L02 cells|
|Western Blot (WB)||WB : 1:20000-1:80000|
|Sample-dependent, check data in validation data gallery|
66543-1-Ig targets SIRT4 in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||SIRT4 fusion protein Ag17347|
|Full Name||sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)|
|Calculated molecular weight||314 aa, 35 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||BC109319|
|Gene ID (NCBI)||23409|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development.
miR-424-5p regulates cell proliferation and migration of esophageal squamous cell carcinoma by targeting SIRT4.
Int J Biochem Cell Biol
SIRT4 regulates rat dental papilla cell differentiation by promoting mitochondrial functions.
Dis Model Mech
Ammonia inhibits energy metabolism in astrocytes in a rapid and glutamate dehydrogenase 2-dependent manner.
20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1α pathway: A translational study.
SETD8 potentiates constitutive ERK1/2 activation via epigenetically silencing DUSP10 expression in pancreatic cancer.