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  • KD/KO Validated

SIRT4 Monoclonal antibody

SIRT4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D5A2

Cat no : 66543-1-Ig

Synonyms

SIR2 like protein 4, SIR2L4, SIRT4



Tested Applications

Positive WB detected inHeLa cells, NCCIT cell, PC-3 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:80000
Sample-dependent, check data in validation data gallery

Product Information

66543-1-Ig targets SIRT4 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIRT4 fusion protein Ag17347
Full Name sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
Calculated molecular weight 314 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC109319
Gene symbol SIRT4
Gene ID (NCBI) 23409
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development.

Protocols

Product Specific Protocols
WB protocol for SIRT4 antibody 66543-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Med

PMID: 31573734

Authors - Jilin Xing
  • KD Validated
humanWB

J Cancer

miR-424-5p regulates cell proliferation and migration of esophageal squamous cell carcinoma by targeting SIRT4.

Authors - Ying Cui
ratWB,IHC,IF

Int J Biochem Cell Biol

SIRT4 regulates rat dental papilla cell differentiation by promoting mitochondrial functions.

Authors - Haoling Chen
  • KD Validated
mouseIHC

Carcinogenesis

SIRT4 is the molecular switch mediating cellular proliferation in colorectal cancer through GLS mediated activation of AKT/GSK3β/CyclinD1 pathway.

Authors - Ying Cui
humanWB

Dis Model Mech

Ammonia inhibits energy metabolism in astrocytes in a rapid and glutamate dehydrogenase 2-dependent manner.

Authors - Leonie Drews
mouseWB

Cell Prolif

20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1α pathway: A translational study.

Authors - Wenxing Cui