SIX1 Monoclonal antibody

SIX1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1


Human, mouse, pig, rabbit, rat







Cat no : 67741-1-Ig


BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX homeobox 1, SIX1, TIP39

Tested Applications

Positive WB detected inL02 cells, HeLa cells, rat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IF detected inA431 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67741-1-Ig targets SIX1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, pig, rabbit, rat samples.

Tested Reactivity Human, mouse, pig, rabbit, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIX1 fusion protein Ag28570
Full Name SIX homeobox 1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight30-35 kDa
GenBank Accession NumberBC008874
Gene Symbol SIX1
Gene ID (NCBI) 6495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.


Product Specific Protocols
WB protocol for SIX1 antibody 67741-1-IgDownload protocol
IF protocol for SIX1 antibody 67741-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols