NKCC1,SLC12A2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NKCC1,SLC12A2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

23

Reviews (2)

Cat no : 13884-1-AP

Synonyms

NKCC1, SLC12A2, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, BSC2, BSC

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Validation Data Gallery

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  WB analysis using 13884-1-AP

  Mouse brain total extract lysate and 37°C incubated mouse brain membrane extract lysate were subjected to SDS PAGE followed by western blot with 13884-1-AP (NKCC1,SLC12A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 13884-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13884-1-AP (NKCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13884-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13884-1-AP (NKCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13884-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13884-1-AP (NKCC1,SLC12A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, 37°C incubated mouse brain tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

For optimal WB detection with 13884-1-AP, we do not recommend boiling the sample after lysis.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 9 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Product Information

13884-1-AP targets NKCC1,SLC12A2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NKCC1,SLC12A2 fusion protein Ag4991
• Full Name: solute carrier family 12 (sodium/potassium/chloride transporters), member 2
• Calculated Molecular Weight: 131 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC033003
• Gene Symbol: NKCC1/SLC12A2
• Gene ID (NCBI): 6558
• RRID: AB_2188522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).

Publications

Species	Application	Title
mouse	WB,IHC	Science; A causative link between inner ear defects and long-term striatal dysfunction.; Authors - Michelle W Antoine
mouse	IHC	PLoS Biol; Early uneven ear input induces long-lasting differences in left-right motor function.; Authors - Michelle W Antoine
mouse	IHC	PLoS Biol; The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner.; Authors - Krisztina Tóth
mouse	WB,IHC	J Neurosci; The severity of vestibular dysfunction in deafness as a determinant of comorbid hyperactivity or anxiety.; Authors - Michelle W Antoine
mouse	IF	Front Mol Neurosci; Unilateral optogenetic kindling of hippocampus leads to more severe impairments of the inhibitory signaling in the contralateral hippocampus; Authors - Fabio Cesar Tescarollo
human	WB	Sci Rep; Comprehensive analyses of solute carrier family members identify SLC12A2 as a novel therapy target for colorectal cancer; Authors - Dan-Yang Chen ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (11-05-2024)

Not sure the marker is actually a positive marker and not a result of the autofluorescence. NKCC1 is a chloride cotransporter that should not be expressed in a fully developed cortex, but it should appear in focal cortical dysplasia tissue used for this assay.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

NKCC1,SLC12A2 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:13884-1-AP)

FH

Valentina (Verified Customer) (02-01-2023)

Multiple bands were detectable around 100 - 150 kDa, with different protocols and gels. The antibody is definitely working even if not optimally, probably necessary to try with a higher dilution and a lower amount of protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Choroid plexus homogenates