Tested Applications
| Positive WB detected in | human brain tissue, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue, human brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19565-1-AP targets KCC2/SLC12A5-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | solute carrier family 12 (potassium-chloride transporter), member 5 |
| Calculated Molecular Weight | 126 kDa |
| Observed Molecular Weight | 130-150 kDa |
| GenBank Accession Number | NM_020708 |
| Gene Symbol | KCC2 |
| Gene ID (NCBI) | 57468 |
| RRID | AB_10638485 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H2X9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KCC2, encoded by SLC12A5, is a neuron-specific K+-Cl- cotransporter that is essential for Cl- homeostasis and inhibitory synaptic transmission in brain. KCC2 is expressed at high levels in neurons throughout the nervous system. KCC2 deletion caused animal death. Alterations of KCC2 have been linked to neuropathic pain, temporal lobe epilepsy, and other diseases. This antibody can detect 130-150 kDa monomer and ~250 kDa dimer of KKC2 in brain lysate. (PMID: 23401525)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KCC2/SLC12A5-Specific antibody 19565-1-AP | Download protocol |
| IHC protocol for KCC2/SLC12A5-Specific antibody 19565-1-AP | Download protocol |
| WB protocol for KCC2/SLC12A5-Specific antibody 19565-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KCC2 antibody (Cat# 19565-1-AP) has 10 citations across journals including Molecular Therapy, Brain Behavior and Immunity, Neurobiology of Disease, iScience, ASN Neuro, Frontiers in Molecular Neuroscience, Ecotoxicology and Environmental Safety, BMC Anesthesiology, and Brain and Behavior. Research fields span GABAergic dysfunction, chloride homeostasis, depression, anxiety, cognitive impairment, hypertension, spinal cord injury, neuropathic pain, and myoclonus, with applications in WB, IF, and IHC.
| Species | Application | Title |
|---|---|---|
Mol Ther Extracellular vesicle-based bumetanide delivery alleviates depression-like behaviors of male mice by restoring chloride homeostasis. | ||
Brain Behav Immun Maternal immune activation induces sex-dependent metabolic, epigenetic and GABAergic trajectories in the hippocampus. | ||
Ecotoxicol Environ Saf 1,2-Dichloroethane causes anxiety and cognitive dysfunction in mice by disturbing GABA metabolism and inhibiting the cAMP-PKA-CREB signaling pathway | ||
Neurobiol Dis HIV and opiates dysregulate K+- Cl- cotransporter 2 (KCC2) to cause GABAergic dysfunction in primary human neurons and Tat-transgenic mice. | ||
iScience Time-restricted feeding entrains long-term behavioral changes through the IGF2-KCC2 pathway. | ||
iScience WNK-SPAK-NKCC1/KCC2 pathway disrupts chloride homeostasis and drives hyperactivation of PVN-CRH neurons in essential hypertension. |
Reviews
What customers say
The single review for this antibody reports a positive experience using it for immunofluorescence on human brain cortex FFPE sections. The reviewer used a 1:50 dilution and observed clear KCC2 staining in green, rating the product 4 out of 5. Overall, the feedback indicates the antibody performed well for IF applications on human tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Reyes (Verified Customer) (03-04-2024) | KCC2 (in green) worked in my IF in human FPPE brain sections
 |
