KCC2/SLC12A5-Specific Polyclonal antibody

KCC2/SLC12A5-Specific Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 19565-1-AP

Synonyms

hKCC2, K Cl cotransporter 2, KCC2, KCC2/SLC12A5-Specific, KIAA1176, Neuronal K Cl cotransporter, SLC12A5

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Validation Data Gallery

• 

  WB analysis using 19565-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19565-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 19565-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19565-1-AP (KCC2/SLC12A5-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse cerebellum using 19565-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using KCC2/SLC12A5-Specific antibody (19565-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 19565-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using KCC2/SLC12A5-Specific antibody (19565-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human brain tissue, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human brain tissue, rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

19565-1-AP targets KCC2/SLC12A5-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: solute carrier family 12 (potassium-chloride transporter), member 5
• Calculated Molecular Weight: 126 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: NM_020708
• Gene Symbol: KCC2
• Gene ID (NCBI): 57468
• RRID: AB_10638485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCC2, encoded by SLC12A5, is a neuron-specific K+-Cl- cotransporter that is essential for Cl- homeostasis and inhibitory synaptic transmission in brain. KCC2 is expressed at high levels in neurons throughout the nervous system. KCC2 deletion caused animal death. Alterations of KCC2 have been linked to neuropathic pain, temporal lobe epilepsy, and other diseases. This antibody can detect 130-150 kDa monomer and ~250 kDa dimer of KKC2 in brain lysate. (PMID: 23401525)

Publications

Species	Application	Title
human,mouse	WB,IF	Neurobiol Dis; HIV and opiates dysregulate K+- Cl- cotransporter 2 (KCC2) to cause GABAergic dysfunction in primary human neurons and Tat-transgenic mice.; Authors - Aaron J Barbour
mouse	IF	iScience; Time-restricted feeding entrains long-term behavioral changes through the IGF2-KCC2 pathway.; Authors - Qiaocheng Zhai
mouse	WB，IF	ASN Neuro; Restoration of KCC2 Membrane Localization in Striatal Dopamine D2 Receptor-Expressing Medium Spiny Neurons Rescues Locomotor Deficits in HIV Tat-Transgenic Mice.; Authors - Aaron J Barbour
rat	WB	Brain Behav; Involvement of the BDNF-TrkB-KCC2 pathway in neuropathic pain after brachial plexus avulsion.; Authors - Shuo Zhao
mouse	IHC	Front Mol Neurosci; Partially brain effects of injection of human umbilical cord mesenchymal stem cells at injury sites in a mouse model of thoracic spinal cord contusion; Authors - Haijun Hu
mouse	WB	Ecotoxicol Environ Saf; 1,2-Dichloroethane causes anxiety and cognitive dysfunction in mice by disturbing GABA metabolism and inhibiting the cAMP-PKA-CREB signaling pathway; Authors - Yuchen Qin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (03-04-2024)

KCC2 (in green) worked in my IF in human FPPE brain sections

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Human Brain Cortex

KCC2/SLC12A5-Specific Antibody Immunofluorescence validation (1:50 dilution) in Human Brain Cortex (Cat no:19565-1-AP)