CoraLite® Plus 488-conjugated KCC2/SLC12A5-Specific Polyclonal antibody

KCC2/SLC12A5-Specific Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19565

Synonyms

hKCC2, K Cl cotransporter 2, KCC2, KIAA1176, Neuronal K Cl cotransporter, SLC12A5

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Validation Data Gallery

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  IF Staining of mouse cerebellum using CL488-19565

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite® Plus 488 KCC2/SLC12A5-Specific antibody (CL488-19565) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse cerebellum tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-19565 targets KCC2/SLC12A5-Specific in IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: solute carrier family 12 (potassium-chloride transporter), member 5
• Calculated Molecular Weight: 126 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: NM_020708
• Gene Symbol: SLC12A5
• Gene ID (NCBI): 57468
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCC2, encoded by SLC12A5, is a neuron-specific K+-Cl- cotransporter that is essential for Cl- homeostasis and inhibitory synaptic transmission in brain. KCC2 is expressed at high levels in neurons throughout the nervous system. KCC2 deletion caused animal death. Alterations of KCC2 have been linked to neuropathic pain, temporal lobe epilepsy, and other diseases. This antibody can detect 130-150 kDa monomer and ~250 kDa dimer of KKC2 in brain lysate. (PMID: 23401525)