Filter: AllWBIHCIF-P

WB analysis using 26804-1-AP (same clone as 26804-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 26804-1-AP (SLC25A12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 26804-1-AP (same clone as 26804-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 26804-1-AP (same clone as 26804-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 26804-1-AP (same clone as 26804-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 26804-1-AP (same clone as 26804-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 26804-1-AP (same clone as 26804-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 26804-1-AP (same clone as 26804-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 26804-1-AP (same clone as 26804-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 26804-1-AP (same clone as 26804-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.
IF Staining of mouse heart using 26804-1-AP (same clone as 26804-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed tissue using 26804-1-AP (SLC25A12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26804-1-PBS in a different storage buffer formulation.

SLC25A12 Polyclonal antibody, PBS Only

SLC25A12 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 26804-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

AGC1, Araceli hiperlarga, Aralar, Aralar1, Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

26804-1-PBS targets SLC25A12 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SLC25A12 fusion protein Ag25150 Predict reactive species
Full Name	solute carrier family 25 (mitochondrial carrier, Aralar), member 12
Calculated Molecular Weight	75 kDa, 63 kDa
Observed Molecular Weight	~70 kDa
GenBank Accession Number	BC016932
Gene Symbol	SLC25A12
Gene ID (NCBI)	8604
RRID	AB_2880641
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75746
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SLC25A12, also known as ARALAR1, is a key component of the malate-aspartate shuttle, a metabolic system that facilitates the transfer of reducing equivalents (NADH) from the cytoplasm into mitochondria, thereby supporting oxidative phosphorylation and ATP production. SLC25A12 dysfunction results in impaired mitochondrial energy production, which can lead to neuronal damage, developmental delays, and myelination defects (PMID: 20015484).