SLC25A20 Polyclonal antibody

SLC25A20 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 19363-1-AP

Synonyms

CAC, CACT, SLC25A20

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Validation Data Gallery

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  WB analysis of mouse heart using 19363-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19363-1-AP (SLC25A20 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19363-1-AP (SLC25A20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 19363-1-AP (SLC25A20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19363-1-AP (SLC25A20 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19363-1-AP (SLC25A20 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19363-1-AP (SLC25A20 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 19363-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19363-1-AP (SLC25A20 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 19363-1-AP

  Immunofluorescent analysis of Hela cells, using SLC25A20 antibody 19363-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of MCF-7 using 19363-1-AP

  Immunofluorescent analysis of MCF-7 cells, using SLC25A20 antibody 19363-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: mouse heart tissue, human heart tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 6 publications below

Product Information

The immunogen of 19363-1-AP is SLC25A20 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC25A20 fusion protein Ag6896
• Full Name: solute carrier family 25 (carnitine/acylcarnitine translocase), member 20
• Calculated molecular weight: 33 kDa
• Observed molecular weight: 33 kDa
• GenBank accession number: BC001689
• Gene symbol: SLC25A20
• Gene ID (NCBI): 788
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.; Authors - Jin-Ho Koh
mouse	WB	Commun Biol; Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism; Authors - Michael Landowski
mouse	WB	Sci Rep; Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.; Authors - Lei Wu
mouse	WB	Front Physiol; PPARδ Attenuates Alcohol-Mediated Insulin Resistance by Enhancing Fatty Acid-Induced Mitochondrial Uncoupling and Antioxidant Defense in Skeletal Muscle.; Authors - Jin-Ho Koh
mouse	WB	Cell Rep; Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.; Authors - Nicole M Sayles
mouse	WB	Am J Physiol Gastrointest Liver Physiol; Medium-chain fatty acid oxidation is independent of L-carnitine in liver and kidney but not in heart and skeletal muscle; Authors - Andrea S Pereyra