SLC25A20 Polyclonal antibody

SLC25A20 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19363-1-AP

Synonyms

CAC, CACT, SLC25A20



Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inmouse heart tissue, human heart tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

The immunogen of 19363-1-AP is SLC25A20 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A20 fusion protein Ag6896
Full Name solute carrier family 25 (carnitine/acylcarnitine translocase), member 20
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC001689
Gene symbol SLC25A20
Gene ID (NCBI) 788
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SLC25A20 antibody 19363-1-APDownload protocol
IHC protocol for SLC25A20 antibody 19363-1-APDownload protocol
IF protocol for SLC25A20 antibody 19363-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.

Authors - Jin-Ho Koh
mouseWB

Commun Biol

Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism

Authors - Michael Landowski
mouseWB

Sci Rep

Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.

Authors - Lei Wu
mouseWB

Front Physiol

PPARδ Attenuates Alcohol-Mediated Insulin Resistance by Enhancing Fatty Acid-Induced Mitochondrial Uncoupling and Antioxidant Defense in Skeletal Muscle.

Authors - Jin-Ho Koh
mouseWB

Cell Rep

Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.

Authors - Nicole M Sayles
mouseWB

Am J Physiol Gastrointest Liver Physiol

Medium-chain fatty acid oxidation is independent of L-carnitine in liver and kidney but not in heart and skeletal muscle

Authors - Andrea S Pereyra