SLC25A4 Polyclonal antibody

SLC25A4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30631-1-AP

Synonyms

AAC1, ADP,ATP carrier protein 1, ADP/ATP translocase 1, ANT, ANT 1, ANT1, PEO2, PEO3, SLC25A4, T1

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Validation Data Gallery

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  WB analysis using 30631-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30631-1-AP (SLC25A4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse stomach using 30631-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 30631-1-AP (SLC25A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 30631-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 30631-1-AP (SLC25A4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: C2C12 cells, mouse brain tissue, mouse heart tissue, mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30631-1-AP targets SLC25A4 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC25A4 fusion protein Ag33385
• Full Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC008664
• Gene Symbol: SLC25A4
• Gene ID (NCBI): 291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2.