SLC26A5 Polyclonal antibody

• 

  WB analysis using 22013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22013-1-AP (SLC26A5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SLC26A5 Polyclonal Antibody for WB, ELISA

Cat No. 22013-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Prestin, PRES, DFNB61

---

Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22013-1-AP targets SLC26A5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC26A5 fusion protein Ag16631
• Full Name: solute carrier family 26, member 5 (prestin)
• Calculated Molecular Weight: 744 aa, 81 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC100832
• Gene Symbol: SLC26A5
• Gene ID (NCBI): 375611
• RRID: AB_3669384
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P58743
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC26A5 (Prestin) is a protein that is housed in abundance within the lateral membrane of outer hair cells (OHC) in the organ of Corti. The protein imparts robust electromechanical activity to the cell that is unlike any other form of cellular motility, notably associated with a voltage-dependent, bell-shaped nonlinear capacitance (NLC) that reports on conformational changes in the protein (PMID: 32061780). SLC26A5 is responsible for acute sensitivity and frequency selectivity in the vertebrate auditory system (PMID: 34294052).