Sodium iodide symporter Polyclonal antibody

Sodium iodide symporter Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

43

Reviews (1)

Cat no : 24324-1-AP

Synonyms

NIS, Sodium-iodide symporter (Na(+)/I(-) symporter), SLC5A5, Natrium iodide transporter

---

Validation Data Gallery

• 

  WB analysis using 24324-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 24324-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 24324-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 24324-1-AP

  IP result of anti-Sodium iodide symporter (IP:24324-1-AP, 4ug; Detection:24324-1-AP 1:500) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human thyroid cancer using 24324-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thyroid cancer using 24324-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 24324-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 24324-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse testis tissue, mouse stomach tissue, SGC-7901 cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human thyroid cancer tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 27 publications below
• IHC: See 7 publications below
• IF: See 14 publications below
• IP: See 1 publications below
• FC: See 2 publications below
• ChIP: See 1 publications below

Product Information

24324-1-AP targets Sodium iodide symporter in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Sodium iodide symporter fusion protein Ag19504
• Full Name: solute carrier family 5 (sodium iodide symporter), member 5
• Calculated Molecular Weight: 643 aa, 69 kDa
• Observed Molecular Weight: 50-55 kDa, 75-100 kDa
• GenBank Accession Number: BC105047
• Gene Symbol: Sodium iodide symporter
• Gene ID (NCBI): 6528
• RRID: AB_2879495
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The sodium iodide symporter (Na+/I - symporter, NIS), encoded by SLC5A5, is an integral plasma membrane glycoprotein that plays an important role in iodide uptake by thyroid cells. Expression of sodium iodide symporter has also been found in extra-thyroidal tissues, including gastric mucosa, lactating mammary gland and salivary glands. Increased expression of sodium iodide symporter has been found in thyroid tissue from patients with Graves' disease as well as papillary thyroid carcinomas. In addition, sodium iodide symporter was found to express in majority of breast cancer tissue but not in normal tissue. Sodium iodide symporter can be a promising diagnostic and therapeutic tool for thyroid cancer and breast cancer. This antibody recognizes the mature approximately 75-100 kDa protein and a partially glycosylated 50-55 kDa protein. (PMID: 12588808, 9525971)

Publications

Species	Application	Title
		Adv Sci (Weinh); Regeneration of Thyroid Glands in the Spleen Restores Homeostasis in Thyroidectomy Mice; Authors - Xue-Jiao Tian
human	WB,IF	Clin Cancer Res; Combined Vorinostat and Chloroquine Inhibit Sodium-Iodide Symporter Endocytosis and Enhance Radionuclide Uptake In Vivo; Authors - Martin L Read
human	WB	Cell Death Differ; REGγ ablation impedes dedifferentiation of anaplastic thyroid carcinoma and accentuates radio-therapeutic response by regulating the Smad7-TGF-β pathway.; Authors - Chan Jiao
human	WB	Cell Death Differ; CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation.; Authors - Yina Liao
human	WB	Thyroid; Dimerization of the sodium/iodide symporter (NIS).; Authors - Rebecca J Thompson
human	WB	Cell Chem Biol; Targeting non-canonical pathways as a strategy to modulate the sodium iodide symporter.; Authors - Martin L Read

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Katie (Verified Customer) (08-22-2019)

Highly specific antibody for the sodium iodide symporter (NIS) with no background bands in Western blot, used at 1:1000 dilution. Very clear result. Good value for money, and excellent communication prior to delivery receipt.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Thyroid carcinoma cell lines

Related Products

TSHR Polyclonal antibody

14450-1-AP

AFP Polyclonal antibody

14550-1-AP

CREB1 Polyclonal antibody

12208-1-AP

PRODH Polyclonal antibody

22980-1-AP