SLC5A6 Polyclonal antibody

• 

  WB analysis of Caco-2 using 26407-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 26407-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of COLO 320 using 26407-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 26407-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 26407-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 26407-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 26407-1-AP (SLC5A6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 26407-1-AP

  IP result of anti-SLC5A6 (IP:26407-1-AP, 4ug; Detection:26407-1-AP 1:500) with mouse brain tissue lysate 5200ug.

SLC5A6 Polyclonal Antibody for WB, IP, ELISA

Cat No. 26407-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

Na(+)-dependent multivitamin transporter, SMVT, Sodium-dependent multivitamin transporter

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Tested Applications

• Positive WB detected in: Caco-2 cells, mouse brain tissue, mouse small intestine tissue, COLO 320 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

26407-1-AP targets SLC5A6 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC5A6 fusion protein Ag23907
• Full Name: solute carrier family 5 (sodium-dependent vitamin transporter), member 6
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC012806
• Gene Symbol: SLC5A6
• Gene ID (NCBI): 8884
• RRID: AB_2880502
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y289
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC5A6, also known as the sodium-dependent multivitamin transporter (hSMVT), is a transmembrane protein that plays a crucial role in the uptake of essential vitamins, including biotin, pantothenic acid, and lipoic acid. This protein is widely expressed in various human tissues, including the placenta, intestine, brain, liver, lung, kidney, cornea, retina, and heart. It uses energy from the transmembrane sodium ion gradient and membrane potential to actively transport these vitamins into cells. Due to different glycosylation modifications, SLC5A6 may exhibit two bands at ~55 kDa and ~69 kDa. (PMID: 20980265, PMID: 25971966)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB	Nat Metab; Vitamin B5 supports MYC oncogenic metabolism and tumor progression in breast cancer; Authors - Peter Kreuzaler
human	WB	Mol Cell Proteomics; Hypoxia is a dominant remodeler of the effector T cell surface proteome relative to activation and regulatory T cell suppression.; Authors - James R Byrnes