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SLC7A5 Polyclonal antibody
SLC7A5 Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (2)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 28670-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, MCF-7 cells, HeLa cells, HepG2 cells, MKN-45 cells, SW480 cells |
Positive IHC detected in | human lung cancer tissue, human placenta tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HT-29 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 5 publications below |
IHC | See 2 publications below |
Product Information
28670-1-AP targets SLC7A5 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SLC7A5 fusion protein Ag29164 |
Full Name | solute carrier family 7 (cationic amino acid transporter, y+ system), member 5 |
Calculated Molecular Weight | 507 aa, 55 kDa |
Observed Molecular Weight | 35-40 kDa |
GenBank Accession Number | BC039692 |
Gene Symbol | SLC7A5 |
Gene ID (NCBI) | 8140 |
RRID | AB_2918188 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for SLC7A5 antibody 28670-1-AP | Download protocol |
IHC protocol for SLC7A5 antibody 28670-1-AP | Download protocol |
IF protocol for SLC7A5 antibody 28670-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Inherit Metab Dis Metabolic characterization of neurogenetic disorders involving glutamatergic neurotransmission | ||
J Exp Clin Cancer Res CircARID1A binds to IGF2BP3 in gastric cancer and promotes cancer proliferation by forming a circARID1A-IGF2BP3-SLC7A5 RNA-protein ternary complex | ||
Front Oncol ZNF24 regulates the progression of KRAS mutant lung adenocarcinoma by promoting SLC7A5 translation | ||
Sci Adv Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells | ||
J Exp Clin Cancer Res FBW7/GSK3β mediated degradation of IGF2BP2 inhibits IGF2BP2-SLC7A5 positive feedback loop and radioresistance in lung cancer |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Xie (Verified Customer) (07-07-2023) | The expression of Slc7a5 is good for my samples
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