EBP50/NHERF-1 Recombinant monoclonal antibody, PBS Only (Detector)

• 

  WB analysis using 87174-1-RR (same clone as 87174-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87174-1-RR (SLC9A3R1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87174-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat kidney using 87174-1-RR (same clone as 87174-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 87174-1-RR (SLC9A3R1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87174-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP02896-1

  The mean EBP50/NHERF-1 concentration was determined to be 2219.3 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 162.0 ng/mL in SH-SY5Y cell extract based on a 1.2 mg/mL extract load.

• 

  Cytometric bead array standard curve of MP02896-1

  Cytometric bead array standard curve of MP02896-1, EBP50/NHERF-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87174-2-PBS. Detection antibody: 87174-1-PBS. Standard: Ag30878. Range: 0.312-40 ng/mL.

• 

  Affinity of 87174-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87174-1-RR against Human EBP50/NHERF-1 were performed. The affinity constant is 2.85 nM.

EBP50/NHERF-1 Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 87174-1-PBS

Clone No.252465A2

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

SLC9A3R1, EBP50, NHERF-1

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Product Information

87174-1-PBS targets EBP50/NHERF-1 as part of a matched antibody pair:

MP02896-1: 87174-2-PBS capture and 87174-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag30878

  Product name: Recombinant human SLC9A3R1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 260-358 aa of NM_004252

  Sequence: TNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL

• Full Name: solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_004252
• Gene Symbol: SLC9A3R1/EBP50/NHERF-1
• Gene ID (NCBI): 9368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14745
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Na+/H+ exchanger regulatory factor 1 (NHERF-1, also known as EBP50 ezrin-binding protein of 50 kDa, SLCA9A3R1) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain. NHERF-1 was initially recognized as a scaffolding protein that recruits membrane transporters/receptors and cytoplasmic signaling proteins into functional complexes localized at or near the plasma membrane in epithelial cells (PMID: 19308292).