SLFN11 Polyclonal antibody

SLFN11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26060-1-AP

Synonyms

Schlafen family member 11, EC:3.1.-.-

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Validation Data Gallery

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  WB analysis using 26060-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26060-1-AP (SLFN11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 26060-1-AP

  IP result of anti-SLFN11 (IP:26060-1-AP, 4ug; Detection:26060-1-AP 1:800) with HEK-293 cells lysate 1610 ug.

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  IHC staining of human ovary cancer using 26060-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26060-1-AP (SLFN11 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 26060-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 26060-1-AP (SLFN11 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: DU 145 cells, HEK-293 cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26060-1-AP targets SLFN11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLFN11 fusion protein Ag22030
• Full Name: schlafen family member 11
• Calculated Molecular Weight: 901 aa, 103 kDa
• Observed Molecular Weight: 103 kDa
• GenBank Accession Number: BC052586
• Gene Symbol: SLFN11
• Gene ID (NCBI): 91607
• RRID: AB_2880356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.