SLN Polyclonal antibody

SLN Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 18395-1-AP

Synonyms

sarcolipin, SLN



Tested Applications

Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

18395-1-AP targets SLN in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, squirrel
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLN fusion protein Ag13176
Full Name sarcolipin
Calculated molecular weight 31 aa, 4 kDa
Observed molecular weight 4 kDa, 8-10kDa
GenBank accession numberBC005261
Gene symbol SLN
Gene ID (NCBI) 6588
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLN, also named as Sarcolipin, belongs to the sarcolipin family. It is associated with calcium ATPase SERCA1. It inhibits SERCA pumps. SLN, a key regulator of cardiac sarco(endo)plasmic reticulum (SR) Ca(2+) ATPase, is predominantly expressed in atria and mediates β-adrenergic responses. SLN can self-assembly to dimer and oligomer for playing importantphysiological function. This antibody recognize the 4kd band in Human heart and skeletal muscle. (PMID: 28241400, PMID: 21737843)

Protocols

Product Specific Protocols
IHC protocol for SLN antibody 18395-1-APDownload protocol
IF protocol for SLN antibody 18395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Physiol Biochem

Sarcolipin expression is repressed by endoplasmic reticulum stress in C2C12 myotubes.

Authors - Nobuhiko Takahashi
squirrelWB,IF

Front Physiol

A Temporal Examination of Cytoplasmic Ca2 + Levels, Sarcoplasmic Reticulum Ca2 + Levels, and Ca2 + -Handling-Related Proteins in Different Skeletal Muscles of Hibernating Daurian Ground Squirrels.

Authors - Zhe Wang
mouseIF

Exp Cell Res

Effect of sarcolipin-mediated cell transdifferentiation in sarcopenia-associated skeletal muscle fibrosis.

Authors - Qiu-Nan Chen
mouseWB

J Endocrinol

ChREBP-β regulates thermogenesis in brown adipose tissue.

Authors - Chunchun Wei

J Biol Chem

Interactions between small ankyrin 1 and sarcolipin coordinately regulate activity of the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA1).

Authors - Patrick F Desmond
mouseWB

Skelet Muscle

Ca(2+) permeation and/or binding to CaV1.1 fine-tunes skeletal muscle Ca(2+) signaling to sustain muscle function.

Authors - Chang Seok Lee