SMAD4 Polyclonal antibody

SMAD4 Polyclonal Antibody for WB, IF, IHC, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG


human, mouse and More (1)


WB, IF, IHC, ELISA, FC (Intra)



Cat no : 51069-2-AP


DPC4, hSMAD4, JIP, MAD homolog 4, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, COLO 320 cells, PC-3 cells, HEK-293 cells, A431 cells, A549 cells, MCF-7 cells
Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue, human heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51069-2-AP targets SMAD4 in WB, IF, IHC, ELISA, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name SMAD family member 4
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC002379
Gene Symbol SMAD4
Gene ID (NCBI) 4089
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interactionwith various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human SMAD4.


Product Specific Protocols
WB protocol for SMAD4 antibody 51069-2-APDownload protocol
IHC protocol for SMAD4 antibody 51069-2-APDownload protocol
IF protocol for SMAD4 antibody 51069-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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