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  • KD/KO Validated

SMARCA4/BRG1 Polyclonal antibody

SMARCA4/BRG1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 21634-1-AP


ATP dependent helicase SMARCA4, BAF190, BAF190A, BRG1, BRG1 associated factor 190A, hSNF2b, Protein brahma homolog 1, Protein BRG 1, SMARCA4, SMARCA4/BRG1, SNF2, SNF2 BETA, SNF2B, SNF2L4, SNF2LB, SWI2, Transcription activator BRG1

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human placenta tissue, mouse brain tissue, rat brain tissue, MCF-7 cells, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue, mouse kidney tissue, human breast cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HEK-293 cells, Hela cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21634-1-AP targets SMARCA4/BRG1 in WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMARCA4/BRG1 fusion protein Ag16256
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Calculated Molecular Weight 1647 aa, 185 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC150298
Gene Symbol SMARCA4
Gene ID (NCBI) 6597
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.


Product Specific Protocols
WB protocol for SMARCA4/BRG1 antibody 21634-1-APDownload protocol
IHC protocol for SMARCA4/BRG1 antibody 21634-1-APDownload protocol
IF protocol for SMARCA4/BRG1 antibody 21634-1-APDownload protocol
IP protocol for SMARCA4/BRG1 antibody 21634-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Clin Oncol

Genomic Characterization of Non-Small-Cell Lung Cancer in African Americans by Targeted Massively Parallel Sequencing.

Authors - Luiz H Araujo

Cell Stem Cell

The long noncoding RNA lncTCF7 promotes self-renewal of human liver cancer stem cells through activation of Wnt signaling.

Authors - Yanying Wang

Mol Cell

SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.

Authors - Shibin Hu

Nucleic Acids Res

Reactivation of the G1 enhancer landscape underlies core circuitry addiction to SWI/SNF

Authors - Katerina Cermakova

Nat Commun

LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells.

Authors - Pingping Zhu

Nucleic Acids Res

HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.

Authors - Xu Zhu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Satyendra (Verified Customer) (07-16-2021)

I have marked Knock out as KO

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK 293
SMARCA4/BRG1 Antibody Western Blot validation (1:1000 dilution) in HEK 293 (Cat no:21634-1-AP)