SMARCA4/BRG1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMARCA4/BRG1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

34

Reviews (1)

Cat no : 21634-1-AP

Synonyms

ATP dependent helicase SMARCA4, BAF190, BAF190A, BRG1, BRG1 associated factor 190A, hSNF2b, Protein brahma homolog 1, Protein BRG 1, SMARCA4, SMARCA4/BRG1, SNF2, SNF2 BETA, SNF2B, SNF2L4, SNF2LB, SWI2, Transcription activator BRG1

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Validation Data Gallery

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  WB analysis of HepG2 using 21634-1-AP

  WB result of SMARCA4/BRG1 antibody (21634-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA4/BRG1 transfected HepG2 cells.

• 

  WB analysis using 21634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21634-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 21634-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21634-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 21634-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21634-1-AP

  IP result of anti-SMARCA4/BRG1 (IP:21634-1-AP, 5ug; Detection:21634-1-AP 1:1000) with HeLa cells lysate 2500ug.

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  IHC staining of human colon cancer using 21634-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21634-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 21634-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human lung cancer using 21634-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human gliomas using 21634-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 21634-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 21634-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 21634-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 21634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 21634-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21634-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21634-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 21634-1-AP

  Immunofluorescent analysis of HepG2 cells, using SMARCA4 antibody 21634-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 21634-1-AP

  Immunofluorescent analysis of Hela cells, using SMARCA4 antibody 21634-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HEK-293 using 21634-1-AP

  Immunofluorescent analysis of 293 cells, using SMARCA4 antibody 21634-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, human placenta tissue, mouse brain tissue, rat brain tissue, MCF-7 cells, PC-3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue, human lung cancer tissue, mouse kidney tissue, human breast cancer tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, HEK-293 cells, Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 21 publications below
• IHC: See 9 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 4 publications below
• ChIP: See 7 publications below
• RIP: See 2 publications below

Product Information

21634-1-AP targets SMARCA4/BRG1 in WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMARCA4/BRG1 fusion protein Ag16256
• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Calculated Molecular Weight: 1647 aa, 185 kDa
• Observed Molecular Weight: 185 kDa
• GenBank Accession Number: BC150298
• Gene Symbol: SMARCA4
• Gene ID (NCBI): 6597
• RRID: AB_10858784
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Publications

Species	Application	Title
human	IHC	J Clin Oncol; Genomic Characterization of Non-Small-Cell Lung Cancer in African Americans by Targeted Massively Parallel Sequencing.; Authors - Luiz H Araujo
human	WB	Cell Stem Cell; The long noncoding RNA lncTCF7 promotes self-renewal of human liver cancer stem cells through activation of Wnt signaling.; Authors - Yanying Wang
	ChIP	Mol Cell; SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.; Authors - Shibin Hu
human	WB,IP	Nucleic Acids Res; Reactivation of the G1 enhancer landscape underlies core circuitry addiction to SWI/SNF; Authors - Katerina Cermakova
human	WB,IHC	Nat Commun; LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells.; Authors - Pingping Zhu
mouse	CoIP	Nucleic Acids Res; HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.; Authors - Xu Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Satyendra (Verified Customer) (07-16-2021)

I have marked Knock out as KO

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK 293

SMARCA4/BRG1 Antibody Western Blot validation (1:1000 dilution) in HEK 293 (Cat no:21634-1-AP)

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