SMCR7/MID49 Polyclonal antibody, PBS Only

• 

  WB analysis using 28718-1-AP (same clone as 28718-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28718-1-AP (SMCR7,MID49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28718-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 28718-1-AP (same clone as 28718-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28718-1-AP (SMCR7,MID49 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28718-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 28718-1-AP (same clone as 28718-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28718-1-AP (SMCR7,MID49 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28718-1-PBS in a different storage buffer formulation.

SMCR7/MID49 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 28718-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

---

Product Information

28718-1-PBS targets SMCR7/MID49 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30119

  Product name: Recombinant human SMCR7,MID49 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 283-454 aa of BC014973

  Sequence: MASEELLLEVQHERLELTVAVLVAVPGVDADDRLLLAWPLEGLAGNLWLQDLYPVEAARLRALDDHDAGTRRRLLLLLCAVCRGCSALGQLGRGHLTQVVLRLGEDNVDWTEEALGERFLQALELLIGSLEQASLPCHFNPSVNLFSSLREEEIDDIGYALYSGLQEPEGLL

• Full Name: Smith-Magenis syndrome chromosome region, candidate 7
• Calculated Molecular Weight: 454 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC014973
• Gene Symbol: MID49
• Gene ID (NCBI): 125170
• RRID: AB_2881198
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96C03
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SMCR7, also named as MID49, is a 49 kDa mitochondrial outer membrane protein which regulates mitochondrial morphology. SMCR7 can directly recruit the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. SMCR7 has two isoforms.