SMCR7/MID49 Polyclonal antibody

SMCR7/MID49 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 28718-1-AP

Synonyms

MID49, SMCR7

---

Validation Data Gallery

• 

  WB analysis using 28718-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28718-1-AP (SMCR7,MID49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 28718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28718-1-AP (SMCR7,MID49 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28718-1-AP (SMCR7,MID49 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: Jurkat cells, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

28718-1-AP targets SMCR7/MID49 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMCR7/MID49 fusion protein Ag30119
• Full Name: Smith-Magenis syndrome chromosome region, candidate 7
• Calculated Molecular Weight: 454 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC014973
• Gene Symbol: SMCR7
• Gene ID (NCBI): 125170
• RRID: AB_2881198
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMCR7, also named as MID49, is a 49 kDa mitochondrial outer membrane protein which regulates mitochondrial morphology. SMCR7 can directly recruit the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. SMCR7 has two isoforms.

Publications

Species	Application	Title
pig	WB	Mol Med; Characterisation of cold-induced mitochondrial fission in porcine aortic endothelial cells.; Authors - Leonard Quiring
mouse	WB	Cell Rep; A PKD-MFF signaling axis couples mitochondrial fission to mitotic progression.; Authors - Evanthia Pangou
human	WB	Nat Commun; Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymes; Authors - Wenjing Yan