Tested Applications
| Positive WB detected in | Jurkat cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28718-1-AP targets SMCR7/MID49 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30119 Product name: Recombinant human SMCR7,MID49 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 283-454 aa of BC014973 Sequence: MASEELLLEVQHERLELTVAVLVAVPGVDADDRLLLAWPLEGLAGNLWLQDLYPVEAARLRALDDHDAGTRRRLLLLLCAVCRGCSALGQLGRGHLTQVVLRLGEDNVDWTEEALGERFLQALELLIGSLEQASLPCHFNPSVNLFSSLREEEIDDIGYALYSGLQEPEGLL Predict reactive species |
| Full Name | Smith-Magenis syndrome chromosome region, candidate 7 |
| Calculated Molecular Weight | 454 aa, 49 kDa |
| Observed Molecular Weight | 49 kDa |
| GenBank Accession Number | BC014973 |
| Gene Symbol | MID49 |
| Gene ID (NCBI) | 125170 |
| RRID | AB_2881198 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96C03 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SMCR7, also named as MID49, is a 49 kDa mitochondrial outer membrane protein which regulates mitochondrial morphology. SMCR7 can directly recruit the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. SMCR7 has two isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SMCR7/MID49 antibody 28718-1-AP | Download protocol |
| WB protocol for SMCR7/MID49 antibody 28718-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-MFF antibody (Cat# 28718-1-AP) has 15 total citations across journals including Nature, Nature Communications, Cell Reports, Free Radical Biology and Medicine, Stem Cell Research & Therapy, Phytomedicine, PLoS Pathogens, Scientific Reports, and others. Research fields span mitochondrial fission, ferroptosis, rheumatoid arthritis, cancer chemotherapy resistance, gastric mucosal injury, pulmonary hypertension, viral proliferation, and neurodegeneration. Applications are predominantly Western blot, with some IHC and IF.
| Species | Application | Title |
|---|---|---|
Nat Commun Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymes | ||
Int J Biol Sci Mitochondrial Dysfunction by FADDosome Promotes Gastric Mucosal Injury in Portal Hypertensive Gastropathy | ||
Phytomedicine Brusatol derivative ameliorates rheumatoid arthritis and atherosclerosis with associated suppression of mitochondrial fission. | ||
Mol Med Characterisation of cold-induced mitochondrial fission in porcine aortic endothelial cells. | ||
Free Radic Biol Med Knockdown of MiD49 and MiD51 alleviates collagen-induced arthritis and suppresses mitophagy and fatty acid oxidation (FAO) in rheumatoid arthritis fibroblast-like synoviocytes
| ||
Clin Transl Med Mitochondrial dysfunction induced by HIF-1α under hypoxia contributes to the development of gastric mucosal lesions |
Reviews
What customers say
Both reviews (2 total) are from the same user and rate the product 5 stars for Western Blot. Reviewers report clear, specific band detection and overall satisfactory performance. No negative feedback or issues were mentioned. Overall sentiment is positive, with users confirming the antibody works as expected in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ziwei (Verified Customer) (01-04-2026) | works as it should be.
|
Ziwei (Verified Customer) (12-14-2025) | Clear and specific bands were detected in Western blot assays.
|
